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PLK5 Polyclonal antibody

PLK5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 21299-1-AP

Print datasheet

Synonyms

PLK5P, polo like kinase 5 pseudogene

验证数据展示

  • WB analysis using 21299-1-AP

    WB analysis using 21299-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21299-1-AP (PLK5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 21299-1-AP

    IF Staining of HeLa using 21299-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PLK5 antibody (21299-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human brain using 21299-1-AP

    IHC staining of human brain using 21299-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 21299-1-AP (PLK5 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human brain using 21299-1-AP

    IHC staining of human brain using 21299-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 21299-1-AP (PLK5 antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inK-562 cells, mouse testis tissue, mouse kidney tissue
Positive IHC detected inhuman brain tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21299-1-AP targets PLK5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen PLK5 fusion protein Ag15881 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name polo-like kinase 5 pseudogene
Synonyms PLK5P, polo like kinase 5 pseudogene
Calculated Molecular Weight 336 aa, 36 kDa
Observed Molecular Weight 55-60 kDa
GenBank Accession NumberBC100792
Gene Symbol PLK5
Gene ID (NCBI) 126520
RRIDAB_10732958
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ496M5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PLK5, also named as PLK5P, is an inactive serine/threonine-protein kinase that plays a role in cell cycle progression and neuronal differentiation. Compared with mouse Plk5, the protein kinase domain of human PLK5 is truncated. This is the reason that the MW of mouse Plk5 is about 50-70 kDa, the MW of human PLK5 is about 32-40 kDa. PLK5 is located in the cytoplasm and nucleus(Uniprot). This antibody detects both PLK5 gene of human and mouse.

实验方案

Product Specific Protocols
WB protocol for PLK5 antibody 21299-1-APDownload protocol
IHC protocol for PLK5 antibody 21299-1-APDownload protocol
IF protocol for PLK5 antibody 21299-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC,IF

Eur J Cell Biol

TWEAK/Fn14 signaling may function as a reactive compensatory mechanism against extracellular matrix accumulation in keloid fibroblasts

Authors - Yitian Zhang
View Article