验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells, mouse testis tissue, mouse kidney tissue |
| Positive IHC detected in | human brain tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy PLK5P Ready-To-Use IHC Kit. PLK5P primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
21299-1-AP targets PLK5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | PLK5 fusion protein Ag15881 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | polo-like kinase 5 pseudogene |
| 别名 | PLK5P, polo like kinase 5 pseudogene |
| 计算分子量 | 336 aa, 36 kDa |
| 观测分子量 | 55-60 kDa |
| GenBank蛋白编号 | BC100792 |
| 基因名称 | PLK5 |
| Gene ID (NCBI) | 126520 |
| RRID | AB_10732958 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q496M5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PLK5, also named as PLK5P, is an inactive serine/threonine-protein kinase that plays a role in cell cycle progression and neuronal differentiation. Compared with mouse Plk5, the protein kinase domain of human PLK5 is truncated. This is the reason that the MW of mouse Plk5 is about 50-70 kDa, the MW of human PLK5 is about 32-40 kDa. PLK5 is located in the cytoplasm and nucleus(Uniprot). This antibody detects both PLK5 gene of human and mouse.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PLK5 antibody 21299-1-AP | Download protocol |
| IHC protocol for PLK5 antibody 21299-1-AP | Download protocol |
| IF protocol for PLK5 antibody 21299-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Eur J Cell Biol TWEAK/Fn14 signaling may function as a reactive compensatory mechanism against extracellular matrix accumulation in keloid fibroblasts | ||
Hum Mol Genet Rare and common genetic variants underlying the risk of Hirschsprung's disease |
