验证数据展示
经过测试的应用
| Positive WB detected in | DU 145 cells, A431 cells, HEK-293 cells, THLE-2 cells, A549 cells, Hela cells, HEK293 cells, HepG2 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Mouse monoclonal antibodies of IgA isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 2 publications below |
| WB | See 4 publications below |
| IHC | See 3 publications below |
产品信息
66342-1-Ig targets PLOD2 in WB, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | PLOD2 fusion protein Ag5779 种属同源性预测 |
| 宿主/亚型 | Mouse / IgA |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | procollagen-lysine, 2-oxoglutarate 5-dioxygenase 2 |
| 别名 | Procollagen-lysine,2-oxoglutarate 5-dioxygenase 2, PLOD2/LH2, Lysyl hydroxylase 2, EC:1.14.11.4, 1H9E1 |
| 计算分子量 | 758 aa, 85 kDa |
| 观测分子量 | 87 kDa |
| GenBank蛋白编号 | BC037169 |
| 基因名称 | PLOD2 |
| Gene ID (NCBI) | 5352 |
| RRID | AB_2881722 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | O00469 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PLOD2,also named as LH2, forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. It is a potential novel prognostic factor for HCC patients following surgery. Among the PLOD genes, PLOD2 contributes to cancer prognosis and angiogenesis. Several authors have reported that PLOD2 expression might provide prognostic information about malignant tumours such as glioblastoma. PLOD2 expression is a useful biomarker for the effects of antiangiogenic treatment for malignancy.(PMID:22098155). It has 2 isoforms produced by alternative splicing and seven glycosylation sites.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PLOD2 antibody 66342-1-Ig | Download protocol |
| IHC protocol for PLOD2 antibody 66342-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Cell Dev Biol Identification and Validation of a Nine-Gene Amino Acid Metabolism-Related Risk Signature in HCC. | ||
J Invest Dermatol Loss of RhoE function in dermatofibroma promotes disorganized dermal fibroblast extracellular matrix and increased integrin activation
| ||
Cell Death Dis Hypoxia-induced PLOD2 promotes clear cell renal cell carcinoma progression via modulating EGFR-dependent AKT pathway activation
| ||
Aging (Albany NY) Systematic characterization of the expression, prognosis and immune characteristics of PLOD family genes in breast cancer |
