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Species Reactivity
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验证数据展示

  • WB analysis of THLE-2 using 66342-1-Ig (same clone as 66342-1-PBS)

    WB analysis of THLE-2 using 66342-1-Ig (same clone as 66342-1-PBS)

    WB result of PLOD2 antibody (66342-1-Ig; 1:15000; room temperature for 1.5 hours) with wild-type and PLOD2 knockout THLE-2 cells. This data was developed using the same antibody clone with 66342-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 66342-1-Ig (same clone as 66342-1-PBS)

    WB analysis of HEK-293 using 66342-1-Ig (same clone as 66342-1-PBS)

    WB result of PLOD2 antibody (66342-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PLOD2 transfected HEK-293 cells. This data was developed using the same antibody clone with 66342-1-PBS in a different storage buffer formulation.

  • WB analysis using 66342-1-Ig (same clone as 66342-1-PBS)

    WB analysis using 66342-1-Ig (same clone as 66342-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66342-1-Ig (PLOD2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66342-1-PBS in a different storage buffer formulation.

  • WB analysis of DU 145 using 66342-1-Ig (same clone as 66342-1-PBS)

    WB analysis of DU 145 using 66342-1-Ig (same clone as 66342-1-PBS)

    DU 145 cells were subjected to SDS PAGE followed by western blot with 66342-1-Ig (PLOD2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66342-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66342-1-Ig (same clone as 66342-1-PBS)

    IHC staining of human liver cancer using 66342-1-Ig (same clone as 66342-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66342-1-Ig (PLOD2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66342-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PLOD2 antibody included
  • IHC staining of human liver cancer using 66342-1-Ig (same clone as 66342-1-PBS)

    IHC staining of human liver cancer using 66342-1-Ig (same clone as 66342-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66342-1-Ig (PLOD2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66342-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PLOD2 antibody included
  • IHC staining of human liver cancer using 66342-1-Ig (same clone as 66342-1-PBS)

    IHC staining of human liver cancer using 66342-1-Ig (same clone as 66342-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66342-1-Ig (PLOD2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66342-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PLOD2 antibody included
  • IHC staining of human liver cancer using 66342-1-Ig (same clone as 66342-1-PBS)

    IHC staining of human liver cancer using 66342-1-Ig (same clone as 66342-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66342-1-Ig (PLOD2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66342-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PLOD2 antibody included
  • Featured Product
  • KD/KO Validated

PLOD2 Monoclonal antibody, PBS Only

PLOD2 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66342-1-PBS

Print datasheet

CloneNo. 1H9E1

Host / Isotype

Mouse / IgA

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

Procollagen-lysine,2-oxoglutarate 5-dioxygenase 2, PLOD2/LH2, Lysyl hydroxylase 2, EC:1.14.11.4, 1H9E1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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Mouse monoclonal antibodies of IgA isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

Planning an IHC experiment? We recommend our IHCeasy PLOD2 Ready-To-Use IHC Kit. PLOD2 primary antibody included.

产品信息

66342-1-PBS targets PLOD2 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen PLOD2 fusion protein Ag5779 种属同源性预测
Host / Isotype Mouse / IgA
Class Monoclonal
Type Antibody
Full Name procollagen-lysine, 2-oxoglutarate 5-dioxygenase 2
Synonyms Procollagen-lysine,2-oxoglutarate 5-dioxygenase 2, PLOD2/LH2, Lysyl hydroxylase 2, EC:1.14.11.4, 1H9E1
Calculated Molecular Weight 758 aa, 85 kDa
Observed Molecular Weight 87 kDa
GenBank Accession NumberBC037169
Gene Symbol PLOD2
Gene ID (NCBI) 5352
RRIDAB_2881722
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO00469
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PLOD2,also named as LH2, forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. It is a potential novel prognostic factor for HCC patients following surgery. Among the PLOD genes, PLOD2 contributes to cancer prognosis and angiogenesis. Several authors have reported that PLOD2 expression might provide prognostic information about malignant tumours such as glioblastoma. PLOD2 expression is a useful biomarker for the effects of antiangiogenic treatment for malignancy.(PMID:22098155). It has 2 isoforms produced by alternative splicing and seven glycosylation sites.