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验证数据展示

  • IF Staining of A431 using CL488-12917

    IF Staining of A431 using CL488-12917

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CoraLite® Plus 488 PLS3 antibody (CL488-12917) at dilution of 1:200.

CoraLite® Plus 488-conjugated PLS3 Polyclonal antibody

PLS3 Polyclonal Antibody for IF/ICC
Cat No. CL488-12917

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Synonyms

Plastin 3, plastin 3 (T isoform), PLS3, T plastin

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inA431 cells
For other applications, we recommend the unconjugated version of this antibody, 12917-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-12917 targets PLS3 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, rat
Immunogen PLS3 fusion protein Ag3574 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name plastin 3 (T isoform)
Synonyms Plastin 3, plastin 3 (T isoform), PLS3, T plastin
Calculated Molecular Weight 630 aa, 71 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC039049
Gene Symbol PLS3
Gene ID (NCBI) 5358
RRIDAB_3672554
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP13797
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PLS3, also named T-plastin and Plastin-3, is an actin-bundling protein found in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia. It plays an important role in leukocyte function. L-plastin plays an important role in leukocyte function, and T-plastin is possibly involved in DNA repair. Both T- and L-plastin are implicated in several diseases, and L-plastin is considered to be a valuable marker for cancer (PMID: 15960882).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 PLS3 antibody CL488-12917Download protocol
Standard Protocols
Click here to view our Standard Protocols