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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated PML Polyclonal antibody

PML Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-21041

Print datasheet

Synonyms

MYL, PML, PP8675, promyelocytic leukemia, RING finger protein 71, RNF71, TRIM19

验证数据展示

  • IF Staining of HeLa using CL488-21041

    IF Staining of HeLa using CL488-21041

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 PML antibody (CL488-21041) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PML Ready-To-Use IHC Kit. PML primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 21041-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-21041 targets PML in IF/ICC applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse
Immunogen PML fusion protein Ag15377 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name promyelocytic leukemia
Synonyms MYL, PML, PP8675, promyelocytic leukemia, RING finger protein 71, RNF71, TRIM19
Calculated Molecular Weight 882 aa, 98 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberBC020994
Gene Symbol PML
Gene ID (NCBI) 5371
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP29590
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PML, also named as MYL, RNF71 and TRIM19, is a member of RBCC/TRIM family of proteins that possess a RING finger domain, B-box, and coiled-coil domain. It functions as tumor suppressor. PML is required for normal, caspase-dependent apoptosis in response to DNA damage, FAS, TNF, or interferons. It plays a role in transcription regulation, DNA damage response, DNA repair and chromatin organization. PML plays a role in processes regulated by retinoic acid, regulation of cell division, terminal differentiation of myeloid precursor cells and differentiation of neural progenitor cells. It regulates PTEN compartmentalization through the inhibition of USP7-mediated deubiquitinylation. A large number of alternative spliced transcripts are synthesized from the PML gene, resulting in a variety of PML proteins ranging in molecular weight from 48-97 kDa (PMID:11704850). And it can be detected as 70-130 kDa or larger due to the modification (especially SUMO) (PMID: 16778193, 22438555).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 PML antibody CL488-21041Download protocol
Standard Protocols
Click here to view our Standard Protocols