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验证数据展示

  • WB analysis of mouse brain using 11252-2-AP

    WB analysis of mouse brain using 11252-2-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 11252-2-AP (PMM1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 11252-2-AP

    IHC staining of human liver cancer using 11252-2-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11252-2-AP (PMM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 11252-2-AP

    IHC staining of human liver cancer using 11252-2-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11252-2-AP (PMM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PMM1 Polyclonal antibody

PMM1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11252-2-AP

Print datasheet

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

phosphomannomutase 1, PMM 1, PMM1, PMMH 22, PMMH22, Sec53

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated

Size: 
50 μL
100 μL
150 μL

¥2200/ 100 μL

浓度: 550 μg/ml

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经过测试的应用

Positive WB detected inmouse brain tissue, human brain tissue, HepG2 cells, NIH/3T3 cells
Positive IHC detected inhuman liver cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

11252-2-AP targets PMM1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen PMM1 fusion protein Ag1782 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phosphomannomutase 1
Synonyms phosphomannomutase 1, PMM 1, PMM1, PMMH 22, PMMH22, Sec53
Calculated Molecular Weight 29 kDa
Observed Molecular Weight 29 kDa
GenBank Accession NumberBC016818
Gene Symbol PMM1
Gene ID (NCBI) 5372
RRIDAB_2166971
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92871
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PMM1, also named as PMMH22, belongs to the eukaryotic PMM family. It catalyzes the second step in the conversion of fructose-6P to GDP-mannoseis and is involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions. In addition, PMM1 may be responsible for the degradation of glucose-1,6-bisphosphate in ischemic brain(PMID:18927083).

实验方案

Product Specific Protocols
WB protocol for PMM1 antibody 11252-2-APDownload protocol
IHC protocol for PMM1 antibody 11252-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

J Proteome Res

Changes in the striatal proteome of YAC128Q mice exhibit gene-environment interactions between mutant huntingtin and manganese.

Authors - Wegrzynowicz Michal M
View Article