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验证数据展示

  • WB analysis using 84894-5-RR (same clone as 84894-5-PBS)

    WB analysis using 84894-5-RR (same clone as 84894-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84894-5-RR (PMS2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84894-5-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 84894-5-RR (same clone as 84894-5-PBS)

    IHC staining of human colon cancer using 84894-5-RR (same clone as 84894-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84894-5-RR (PMS2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84894-5-PBS in a different storage buffer formulation.

  • FC experiment of MCF-7 using 84894-5-RR (same clone as 84894-5-PBS)

    FC experiment of MCF-7 using 84894-5-RR (same clone as 84894-5-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug PMS2 Recombinant antibody (84894-5-RR, Clone:242455F8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84894-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84894-5-RR

    Affinity and Kinetic Characterization of 84894-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84894-5-RR against Human PMS2 were performed. The affinity constant is below 1 pM.

PMS2 Recombinant antibody, PBS Only

PMS2 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), Indirect ELISA
Cat No. 84894-5-PBS

产品说明书

CloneNo. 242455F8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, FC (Intra), Indirect ELISA

别名

PMSL2, PMS2CL, PMS1 protein homolog 2, HNPCC4, EC:3.1.-.-

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84894-5-PBS targets PMS2 in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 PMS2 fusion protein Ag33508 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 PMS2 postmeiotic segregation increased 2 (S. cerevisiae)
别名 PMSL2, PMS2CL, PMS1 protein homolog 2, HNPCC4, EC:3.1.-.-
计算分子量 862 aa, 96 kDa
观测分子量117 kDa
GenBank蛋白编号BC093921
基因名称 PMS2
Gene ID (NCBI) 5395
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP54278
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PMS2, also named as PMSL2, belongs to the DNA mismatch repair mutL/hexB family. It is a component of the post-replicative DNA mismatch repair system (MMR). It heterodimerizes with MLH1 to form MutL alpha. MulL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. It also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. (PMID: 16873062, PMID: 18206974) Defects in PMS2 are the cause of hereditary non-polyposis colorectal cancer type 4 (HNPCC4). Defects in PMS2 are a cause of mismatch repair cancer syndrome (MMRCS).