CoraLite®594-conjugated PNPLA3 Monoclonal antibody

PNPLA3 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1D2B11

Cat no : CL594-67369

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Synonyms

Acylglycerol O acyltransferase, Adiponutrin, ADPN, C22orf20, iPLA(2)epsilon, iPLA2 epsilon, PNPLA3



经过测试的应用

Positive IF/ICC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67369-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67369 targets PNPLA3 in IF/ICC applications and shows reactivity with Human, Rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human, Rat
Immunogen PNPLA3 fusion protein Ag27407 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name patatin-like phospholipase domain containing 3
Synonyms Acylglycerol O acyltransferase, Adiponutrin, ADPN, C22orf20, iPLA(2)epsilon, iPLA2 epsilon, PNPLA3
Observed Molecular Weight 53 kDa
GenBank Accession NumberBC065195
Gene Symbol PNPLA3
Gene ID (NCBI) 80339
RRIDAB_2920121
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9NST1
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PNPLA3 belongs to a family of proteins that share a domain that was first identified in patatin, a major soluble protein of potato tubers with nonspecific acyl hydrolase activity. The domain differs from classical lipases by employing a catalytic dyad (Ser-Asp), rather than a catalytic triad, to effect hydrolysis. PNPLA3 is expressed primarily in liver and adipose tissue, in which it partitions to membranes and lipid droplets. Real-time PCR of cDNA from human tissues indicated that PNPLA3 expression was highest in the liver, followed by skin and adipose tissue. PNPLA3 is expressed at the highest levels in adipose tissue of mice. The PNPLA3 protein was expected at 50-70 kDa, as observed; the additional band at approximately 130-150 kDa is a oligomer bands. (PMID: 20385813, PMID: 24931521, PMID: 23023705)

实验方案

Product Specific Protocols
IF protocol for CL594 PNPLA3 antibody CL594-67369Download protocol
Standard Protocols
Click here to view our Standard Protocols