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PNPLA7 Polyclonal antibody

PNPLA7 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 27787-1-AP

Print datasheet

Synonyms

RP11 48C7.2, NTEL1, NTE R1, C9orf111

验证数据展示

  • IHC staining of mouse cerebellum using 27787-1-AP

    IHC staining of mouse cerebellum using 27787-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 27787-1-AP (PNPLA7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse cerebellum using 27787-1-AP

    IHC staining of mouse cerebellum using 27787-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 27787-1-AP (PNPLA7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27787-1-AP targets PNPLA7 in IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen PNPLA7 fusion protein Ag27184 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name patatin-like phospholipase domain containing 7
Synonyms RP11 48C7.2, NTEL1, NTE R1, C9orf111
Calculated Molecular Weight 146 kDa
GenBank Accession NumberNM_001098537
Gene Symbol PNPLA7
Gene ID (NCBI) 375775
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6ZV29
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PNPLA7 (Patatin-like phospholipase domain-containing protein 7), also termed NTE-related 1 (NTE-R1) or NRE, is a member of the PNPLAs family, which is conserved protein in mice, rats and humans. It serves key roles in triglyceride hydrolysis, energy metabolism, lipid droplet (LD), and in regulation of adipocyte differentiation. It is reported that PNPLA7 was down-regulated in HCC through hypermethylation of its promoter, which indicates that PNPLA7 may be a potential biomarker for HCC diagnosis (PMID: 16799181, 27347198).

实验方案

Product Specific Protocols
IHC protocol for PNPLA7 antibody 27787-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols