验证数据展示
经过测试的应用
| Positive WB detected in | HCT 116 cells, HUVEC cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human liver cancer tissue, mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HUVEC cells, A431 cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14929-1-AP targets POFUT1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | POFUT1 fusion protein Ag6710 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | protein O-fucosyltransferase 1 |
| 别名 | O FucT 1, O Fuc T, KIAA0180, GDP-fucose protein O-fucosyltransferase 1, FUT12 |
| 计算分子量 | 44 kDa |
| 观测分子量 | 44 kDa |
| GenBank蛋白编号 | BC000582 |
| 基因名称 | POFUT1 |
| Gene ID (NCBI) | 23509 |
| RRID | AB_2166123 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9H488 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
POFUT1 (protein O-fucosyltransferase 1), also known as GDP-fucose protein O-fucosyltransferase, is an enzyme that catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue in EGF domains and plays a crucial role in Notch signaling.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for POFUT1 antibody 14929-1-AP | Download protocol |
| IHC protocol for POFUT1 antibody 14929-1-AP | Download protocol |
| IF protocol for POFUT1 antibody 14929-1-AP | Download protocol |
| IP protocol for POFUT1 antibody 14929-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell In vivo CRISPR screening reveals nutrient signaling processes underpinning CD8+ T cell fate decisions.
| ||
EBioMedicine PLAGL2 and POFUT1 are regulated by an evolutionarily conserved bidirectional promoter and are collaboratively involved in colorectal cancer by maintaining stemness.
| ||
EMBO Mol Med A spoonful of L-fucose-an efficient therapy for GFUS-CDG, a new glycosylation disorder | ||
Cell Death Dis POFUT1 promotes colorectal cancer development through the activation of Notch1 signaling.
| ||
Cell Death Dis LIF upregulates poFUT1 expression and promotes trophoblast cell migration and invasion at the fetal-maternal interface. | ||
Cell Prolif Epiregulin promotes trophoblast epithelial-mesenchymal transition through poFUT1 and O-fucosylation by poFUT1 on uPA. |
