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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated POFUT1 Polyclonal antibody

POFUT1 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-14929

Print datasheet

Synonyms

O FucT 1, O Fuc T, KIAA0180, GDP-fucose protein O-fucosyltransferase 1, FUT12

验证数据展示

  • IF Staining of HUVEC using CL594-14929

    IF Staining of HUVEC using CL594-14929

    Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using CoraLite®594 POFUT1 antibody (CL594-14929) at dilution of 1:200.

  • FC experiment of A431 using CL594-14929

    FC experiment of A431 using CL594-14929

    1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human POFUT1 (CL594-14929) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHUVEC cells, HepG2 cells
Positive FC (Intra) detected inA431 cells
For other applications, we recommend the unconjugated version of this antibody, 14929-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-14929 targets POFUT1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen POFUT1 fusion protein Ag6710 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein O-fucosyltransferase 1
Synonyms O FucT 1, O Fuc T, KIAA0180, GDP-fucose protein O-fucosyltransferase 1, FUT12
Calculated Molecular Weight 44 kDa
Observed Molecular Weight 44 kDa
GenBank Accession NumberBC000582
Gene Symbol POFUT1
Gene ID (NCBI) 23509
RRIDAB_2919815
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H488
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

POFUT1 (protein O-fucosyltransferase 1), also known as GDP-fucose protein O-fucosyltransferase, is an enzyme that catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue in EGF domains and plays a crucial role in Notch signaling.

实验方案

Product Specific Protocols
IF protocol for CL594 POFUT1 antibody CL594-14929Download protocol
FC protocol for CL594 POFUT1 antibody CL594-14929Download protocol
Standard Protocols
Click here to view our Standard Protocols