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POGZ Polyclonal antibody, PBS Only

POGZ Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 30106-1-PBS

Print datasheet

Synonyms

验证数据展示

  • WB analysis using 30106-1-AP (same clone as 30106-1-PBS)

    WB analysis using 30106-1-AP (same clone as 30106-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30106-1-AP (POGZ antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30106-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 30106-1-AP (same clone as 30106-1-PBS)

    IHC staining of mouse brain using 30106-1-AP (same clone as 30106-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30106-1-AP (POGZ antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30106-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with POGZ antibody included
  • IF Staining of HepG2 using 30106-1-AP (same clone as 30106-1-PBS)

    IF Staining of HepG2 using 30106-1-AP (same clone as 30106-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using POGZ antibody (30106-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 30106-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 30106-1-AP (same clone as 30106-1-PBS)

    IP experiment of HeLa using 30106-1-AP (same clone as 30106-1-PBS)

    IP result of anti-POGZ (IP:30106-1-AP, 4ug; Detection:30106-1-AP 1:2000) with HeLa cells lysate 1800 ug. This data was developed using the same antibody clone with 30106-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 30106-1-AP (same clone as 30106-1-PBS)

    IHC staining of mouse testis using 30106-1-AP (same clone as 30106-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30106-1-AP (POGZ antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30106-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with POGZ antibody included
  • IHC staining of mouse testis using 30106-1-AP (same clone as 30106-1-PBS)

    IHC staining of mouse testis using 30106-1-AP (same clone as 30106-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30106-1-AP (POGZ antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30106-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with POGZ antibody included
  • IHC staining of mouse brain using 30106-1-AP (same clone as 30106-1-PBS)

    IHC staining of mouse brain using 30106-1-AP (same clone as 30106-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30106-1-AP (POGZ antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30106-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with POGZ antibody included
  • IF Staining of U-251 using 30106-1-AP (same clone as 30106-1-PBS)

    IF Staining of U-251 using 30106-1-AP (same clone as 30106-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using POGZ antibody (30106-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 30106-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy POGZ Ready-To-Use IHC Kit. POGZ primary antibody included.

产品信息

30106-1-PBS targets POGZ in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen POGZ fusion protein Ag32435 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name pogo transposable element with ZNF domain
Synonyms
Calculated Molecular Weight155kd
Observed Molecular Weight 155 kDa
GenBank Accession NumberNM_015100
Gene Symbol POGZ
Gene ID (NCBI) 23126
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z3K3
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

POGZ, also known as MRD37, is a zinc-finger protein containing 9 C2H2 zinc fingers, a CENP-B (centromere protein-B) domain, and a DDE domain found in the bacterial transposase/retroviral integrase family. POGZ plays a role in mitotic cell cycle progression and is involved in kinetochore assembly and mitotic sister chromatid cohesion. POGZ plays a role in mitotic chromosome segregation by regulating aurora kinase B/AURKB activation and AURKB and CBX5 dissociation from chromosome arms (PMID:20562864). Heterozygous mutation of POGZ causes White-Sutton syndrome (PMID: 26739615). Expect a band approximately 155 kDa in size corresponding to POGZ by western blotting.