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POLR2A Recombinant antibody, PBS Only

POLR2A Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, monkey

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240869E9

Cat no : 84357-1-PBS

Print datasheet

Synonyms

EC:2.7.7.48, DNA-directed RNA polymerase III largest subunit, DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase II subunit A, 3'-5' exoribonuclease

验证数据展示

  • WB analysis of COS-7 using 84357-1-RR (same clone as 84357-1-PBS)

    WB analysis of COS-7 using 84357-1-RR (same clone as 84357-1-PBS)

    COS-7 cells were subjected to SDS PAGE followed by western blot with 84357-1-RR (POLR2A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84357-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 84357-1-RR (same clone as 84357-1-PBS)

    IF Staining of HepG2 using 84357-1-RR (same clone as 84357-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using POLR2A antibody (84357-1-RR, Clone: 240869E9 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84357-1-PBS in a different storage buffer formulation.

产品信息

84357-1-PBS targets POLR2A in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, monkey samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, monkey
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
Synonyms EC:2.7.7.48, DNA-directed RNA polymerase III largest subunit, DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase II subunit A, 3'-5' exoribonuclease
Calculated Molecular Weight 217 kDa
Observed Molecular Weight245 kDa
GenBank Accession NumberNM_000937
Gene Symbol POLR2A
Gene ID (NCBI) 5430
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP24928
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DNA-directed RNA polymerase II subunit RPB1(POLR2A), is part of the core element of the basic RNA polymerase II transcription mechanism. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for the assembly of factors that regulate transcription initiation, elongation, termination, and mRNA processing.