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POLR2H Recombinant antibody, PBS Only (Detector)

POLR2H Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA, Cytometric bead array

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, Indirect ELISA, Cytometric bead array

Conjugate

Unconjugated

CloneNo.

240410F9

Cat no : 83439-1-PBS

Print datasheet

Synonyms

hsRPB8, hRPB8, DNA-directed RNA polymerases I, II, and III subunit RPABC3, DNA-directed RNA polymerases I, II, and III 17.1 kDa polypeptide, DNA-directed RNA polymerase II subunit H

验证数据展示

  • WB analysis using 83439-1-RR (same clone as 83439-1-PBS)

    WB analysis using 83439-1-RR (same clone as 83439-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83439-1-RR (POLR2H antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83439-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00447-1

    Cytometric bead array standard curve of MP00447-1

    Cytometric bead array standard curve of MP00447-1, POLR2H Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83439-2-PBS. Detection antibody: 83439-1-PBS. Standard: Ag7166. Range: 0.78-100 ng/mL.

  • Affinity and Kinetic Characterization of 83439-1-PBS

    Affinity and Kinetic Characterization of 83439-1-PBS

    Biolayer interferometry (BLl) kinetic assays of 83439-1-RR against Human POLR2H were performed. The affinity constant is below 1 pM.

产品信息

83439-1-PBS targets POLR2H as part of a matched antibody pair:

MP00447-1: 83439-2-PBS capture and 83439-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, Indirect ELISA, Cytometric bead array Application Description
Tested Reactivity Human
Immunogen POLR2H fusion protein Ag7166 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name polymerase (RNA) II (DNA directed) polypeptide H
Synonyms hsRPB8, hRPB8, DNA-directed RNA polymerases I, II, and III subunit RPABC3, DNA-directed RNA polymerases I, II, and III 17.1 kDa polypeptide, DNA-directed RNA polymerase II subunit H
Calculated Molecular Weight 17 kDa
GenBank Accession NumberBC000739
Gene Symbol POLR2H
Gene ID (NCBI) 5437
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP52434
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C