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  • Featured Product
  • KD/KO Validated

POLR3D Monoclonal antibody, PBS Only

POLR3D Monoclonal Antibody for Indirect ELISA, WB

Host / Isotype

Mouse / IgG1

Reactivity

rabbit, human

Applications

Indirect ELISA, WB

Conjugate

Unconjugated

CloneNo.

2F5G4

Cat No : 68263-1-PBS

Print datasheet

Synonyms

RPC4, DNA-directed RNA polymerase III subunit RPC4, DNA-directed RNA polymerase III subunit D, BN51T, BN51

验证数据展示

  • WB analysis using 68263-1-Ig (same clone as 68263-1-PBS)

    WB analysis using 68263-1-Ig (same clone as 68263-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68263-1-Ig (POLR3D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68263-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 68263-1-Ig (same clone as 68263-1-PBS)

    WB analysis of HEK-293 using 68263-1-Ig (same clone as 68263-1-PBS)

    WB result of POLR3D antibody (68263-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-POLR3D transfected HEK-293 cells. This data was developed using the same antibody clone with 68263-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 68263-1-Ig (same clone as 68263-1-PBS)

    FC experiment of HeLa using 68263-1-Ig (same clone as 68263-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug POLR3D Monoclonal antibody (68263-1-Ig, Clone:2F5G4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 68263-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy POLR3D Ready-To-Use IHC Kit. POLR3D primary antibody included.

产品信息

68263-1-PBS targets POLR3D in Indirect ELISA, WB applications and shows reactivity with rabbit, human samples.

Tested Applications Indirect ELISA, WB Application Description
Tested Reactivity rabbit, human
Immunogen POLR3D fusion protein Ag7646 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name polymerase (RNA) III (DNA directed) polypeptide D, 44kDa
Synonyms RPC4, DNA-directed RNA polymerase III subunit RPC4, DNA-directed RNA polymerase III subunit D, BN51T, BN51
Calculated Molecular Weight 44 kDa
Observed Molecular Weight44 kDa
GenBank Accession NumberBC004484
Gene Symbol POLR3D
Gene ID (NCBI) 661
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP05423
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Polymerase (RNA) III (DNA directed) polypeptide D(POLR3D) plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses and acts as nuclear and cytosolic DNA sensor involved in innate immune response. POLR3D can sense non-self dsDNA that serves as template for transcription into dsRNA.