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PON1 Recombinant antibody, PBS Only (Capture)

PON1 Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241091B7

Cat no : 83982-2-PBS

Print datasheet

Synonyms

paraoxonase 1, K-45, EC:3.1.1.2, Aromatic esterase 1, A-esterase 1

验证数据展示

  • WB analysis using 83982-2-RR (same clone as 83982-2-PBS)

    WB analysis using 83982-2-RR (same clone as 83982-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83982-2-RR (PON1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83982-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00877-2

    Cytometric bead array standard curve of MP00877-2

    Cytometric bead array standard curve of MP00877-2, PON1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83982-2-PBS. Detection antibody: 83982-1-PBS. Standard: Ag12934. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 83982-2-RR

    Affinity and Kinetic Characterization of 83982-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83982-2-RR against Human PON1 were performed. The affinity constant is below 1 pM.

Planning an IHC experiment? We recommend our IHCeasy PON1 Ready-To-Use IHC Kit. PON1 primary antibody included.

产品信息

83982-2-PBS targets PON1 as part of a matched antibody pair:

MP00877-2: 83982-2-PBS capture and 83982-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human
Immunogen PON1 fusion protein Ag12934 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name paraoxonase 1
Synonyms paraoxonase 1, K-45, EC:3.1.1.2, Aromatic esterase 1, A-esterase 1
Calculated Molecular Weight 355 aa, 40 kDa
Observed Molecular Weight40-45 kDa
GenBank Accession NumberBC074719
Gene Symbol PON1
Gene ID (NCBI) 5444
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP27169
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PON1, also named PON and K-45, belongs to the paraoxonase family. PON1 hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. It is capable of hydrolyzing a broad spectrum of organophosphate substrates and several aromatic carboxylic acid esters. PON1 may mediate enzymatic protection of low-density lipoproteins against oxidative modification and the consequent series of events leading to atheroma formation. PON1 was identified as a triple band in a range of approximately 35-40 kDa. For glycosylated, the MW of PON1 is migrated 42-45kd, while the 35 kDa band represents the unglycosylated form (PMID: 17906223, 32002976, 21852972). The antibody has cross-reaction to PON2 and PON3.