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验证数据展示

  • WB analysis using 84604-4-RR

    WB analysis using 84604-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84604-4-RR (PON3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 84604-4-RR

    IHC staining of mouse liver using 84604-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84604-4-RR (PON3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84604-4-RR

    Affinity and Kinetic Characterization of 84604-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84604-4-RR against Human PON3 were performed. The affinity constant is below 1 pM.

PON3 Recombinant antibody

PON3 Recombinant Antibody for WB, IHC, ELISA
Cat No. 84604-4-RR

产品说明书

CloneNo. 242035D11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

Paraoxonase 3 , Paraoxanase-3, EC:3.1.8.1, 242035D11

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HuH-7 cells, HepG2 cells
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84604-4-RR targets PON3 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 PON3 fusion protein Ag36602 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 paraoxonase 3
别名 Paraoxonase 3 , Paraoxanase-3, EC:3.1.8.1, 242035D11
计算分子量NM_000940.2 40kDa，354aa
观测分子量40 kDa
GenBank蛋白编号NM_000940.2
基因名称 PON3
Gene ID (NCBI) 5446
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ15166
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PON3, a member of the paraoxonase family, is expressed primarily in the liver and encodes a protein that is secreted into the bloodstream and binds to high-density lipoprotein (HDL). It also rapidly hydrolyzes lactones and inhibits the oxidation of low-density lipoprotein (LDL), thereby slowing the onset and progression of atherosclerosis. In addition, PON3 is closely associated with hepatocellular carcinoma (HCC), and is a potential therapeutic target for HCC by inducing cell cycle arrest and further inhibiting HCC cell proliferation.

实验方案

Product Specific Protocols
WB protocol for PON3 antibody 84604-4-RRDownload protocol
IHC protocol for PON3 antibody 84604-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols