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POT1 Polyclonal antibody, PBS Only

POT1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 10581-1-PBS

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Synonyms

验证数据展示

  • WB analysis of HeLa using 10581-1-AP (same clone as 10581-1-PBS)

    WB analysis of HeLa using 10581-1-AP (same clone as 10581-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 10581-1-AP (POT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10581-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 10581-1-AP (same clone as 10581-1-PBS)

    IHC staining of human prostate cancer using 10581-1-AP (same clone as 10581-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10581-1-AP (POT1 antibody) at dilution of 1:100 (under 25x lens). This data was developed using the same antibody clone with 10581-1-PBS in a different storage buffer formulation.

产品信息

10581-1-PBS targets POT1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen POT1 fusion protein Ag0875 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name POT1 protection of telomeres 1 homolog (S. pombe)
Synonyms
Calculated Molecular Weight 71 kDa
Observed Molecular Weight 45 kDa, 71 kDa
GenBank Accession NumberBC002923
Gene Symbol POT1
Gene ID (NCBI) 25913
RRIDAB_2284057
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NUX5
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Protection of telomeres 1(POT1) is a conserved protein that binds the G-rich stand of its own telomeric repeat sequence, with a role in protecting chromosome ends. It's housekeeping gene that is required to ensure the integrity of chromosome ends in all cells. It's a component of the telomerase ribonucleoprotein(RNP) complex that is esential for the replication of chromosome termini. It consists the TRF1 compex that is involved in the regulation of telomere length by cis-inhibition of telomerase. We have discovered that POT1 exists in at least three consistently occurring forms; 90, 70 and 45kDa. The unexpected molecular weights of POT1 seem to be associated with SUMO1 and ubiquitin conjugation; the latter occurring at a double lysine residue at 289-KK-290 (.PMID: 24054699)