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Species Reactivity
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Conjugate
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Conjugate
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验证数据展示

  • WB analysis of HeLa using 10581-1-AP (same clone as 10581-1-PBS)

    WB analysis of HeLa using 10581-1-AP (same clone as 10581-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 10581-1-AP (POT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10581-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 10581-1-AP (same clone as 10581-1-PBS)

    IHC staining of human prostate cancer using 10581-1-AP (same clone as 10581-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10581-1-AP (POT1 antibody) at dilution of 1:100 (under 25x lens). This data was developed using the same antibody clone with 10581-1-PBS in a different storage buffer formulation.

POT1 Polyclonal antibody, PBS Only

POT1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 10581-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

10581-1-PBS targets POT1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 POT1 fusion protein Ag0875 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 POT1 protection of telomeres 1 homolog (S. pombe)
别名
计算分子量 71 kDa
观测分子量 45 kDa, 71 kDa
GenBank蛋白编号BC002923
基因名称 POT1
Gene ID (NCBI) 25913
RRIDAB_2284057
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NUX5
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Protection of telomeres 1(POT1) is a conserved protein that binds the G-rich stand of its own telomeric repeat sequence, with a role in protecting chromosome ends. It's housekeeping gene that is required to ensure the integrity of chromosome ends in all cells. It's a component of the telomerase ribonucleoprotein(RNP) complex that is esential for the replication of chromosome termini. It consists the TRF1 compex that is involved in the regulation of telomere length by cis-inhibition of telomerase. We have discovered that POT1 exists in at least three consistently occurring forms; 90, 70 and 45kDa. The unexpected molecular weights of POT1 seem to be associated with SUMO1 and ubiquitin conjugation; the latter occurring at a double lysine residue at 289-KK-290 (.PMID: 24054699)