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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 85096-1-RR (same clone as 85096-1-PBS)

    WB analysis using 85096-1-RR (same clone as 85096-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85096-1-RR (PPAT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85096-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85096-1-RR

    Affinity and Kinetic Characterization of 85096-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85096-1-RR against Human PPAT were performed. The affinity constant is 9.40 nM.

PPAT Recombinant antibody, PBS Only

PPAT Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 85096-1-PBS

产品说明书

CloneNo. 242513C4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, Indirect ELISA

别名

PRAT, phosphoribosyl pyrophosphate amidotransferase, GPAT, EC:2.4.2.14, 242513C4

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

85096-1-PBS targets PPAT in WB, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human
免疫原 PPAT fusion protein Ag7618 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 phosphoribosyl pyrophosphate amidotransferase
别名 PRAT, phosphoribosyl pyrophosphate amidotransferase, GPAT, EC:2.4.2.14, 242513C4
计算分子量 57 kDa
观测分子量57 kDa
GenBank蛋白编号BC004200
基因名称 PPAT
Gene ID (NCBI) 5471
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ06203
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PPAT, also known as ATase (amidophosphoribosyltransferase) or GPAT (glutamine phosphoribosylpyrophosphate amidotransferase) is the rate-limiting enzyme in the de novo pathway of purine ribonucleotide synthesis and is regulated by feedback inhibition by AMP and GMP. PPAT expression modulates pyruvate kinase activity, cell proliferation and invasion (PMID: 26140362).