PPAT Recombinant antibody

PPAT Recombinant Antibody for WB, ELISA
Cat No. 85096-1-RR
CloneNo. 242513C4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -


经过测试的应用

Positive WB detected inA549 cells, MCF-7 cells, LNCaP cells, HEK-293 cells, HepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85096-1-RR targets PPAT in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen PPAT fusion protein Ag7618 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name phosphoribosyl pyrophosphate amidotransferase
Synonyms
Calculated Molecular Weight 57 kDa
Observed Molecular Weight57 kDa
GenBank Accession NumberBC004200
Gene Symbol PPAT
Gene ID (NCBI) 5471
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ06203
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PPAT, also known as ATase (amidophosphoribosyltransferase) or GPAT (glutamine phosphoribosylpyrophosphate amidotransferase) is the rate-limiting enzyme in the de novo pathway of purine ribonucleotide synthesis and is regulated by feedback inhibition by AMP and GMP. PPAT expression modulates pyruvate kinase activity, cell proliferation and invasion (PMID: 26140362).

实验方案

Product Specific Protocols
WB protocol for PPAT antibody 85096-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
Loading...