验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, HepG2 cells, human liver tissue, rat kidney tissue, mouse kidney tissue, K-562 cells |
| Positive IHC detected in | human breast cancer tissue, human urothelial carcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy PPM1A Ready-To-Use IHC Kit. PPM1A primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IF | See 1 publications below |
产品信息
12961-1-AP targets PPM1A in WB, IF, IHC, ELISA, Cell treatment applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IF, Cell treatment |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse, rat |
| Immunogen | PPM1A fusion protein Ag3645 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | protein phosphatase 1A (formerly 2C), magnesium-dependent, alpha isoform |
| Synonyms | PP2C ALPHA, PP2CA, PPM1A, PPPM1A, Protein phosphatase 1A, Protein phosphatase IA |
| Calculated Molecular Weight | 382 aa, 42 kDa |
| Observed Molecular Weight | 42 kDa |
| GenBank Accession Number | BC026691 |
| Gene Symbol | PPM1A |
| Gene ID (NCBI) | 5494 |
| RRID | AB_2169763 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P35813 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PPM1A(Protein phosphatase 1A) is also named as PPPM1A and belongs to the PP2C family. It is a serine/threonine protein phosphatase that is essential for regulating cellular stress responses in eukaryotes. PPM1A plays an important role in tumor suppression and that it negatively controls many signaling pathways which are critical for trophoblast invasion.(PMID:19404668). This protein distributes in both the cytoplasm and nucleus of mammalian cells(PMID:9003755). It has 2 isoforms produced by alternative splicing.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PPM1A antibody 12961-1-AP | Download protocol |
| IHC protocol for PPM1A antibody 12961-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Rep Blocking AMPK β1 myristoylation enhances AMPK activity and protects mice from high-fat diet-induced obesity and hepatic steatosis | ||
iScience Induced retinal pigment epithelial cells with anti-epithelial-to-mesenchymal transition ability delay retinal degeneration | ||
Biol Pharm Bull Sorghum (Sorghum bicolor) Extract-Induced Adipogenesis Is Independent of PPARγ Ser273 Phosphorylation in 3T3-L1 Adipocytes. |
