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验证数据展示

  • WB analysis using 67647-1-Ig

    WB analysis using 67647-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67647-1-Ig (PPM1B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis using 67647-1-Ig

    WB analysis using 67647-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67647-1-Ig (PPM1B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 67647-1-Ig

    IHC staining of human breast cancer using 67647-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67647-1-Ig (PPM1B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 67647-1-Ig

    IHC staining of human breast cancer using 67647-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67647-1-Ig (PPM1B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PPM1B Monoclonal antibody

PPM1B Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67647-1-Ig

产品说明书

CloneNo. 2C7H2

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat

应用

WB, IHC, ELISA

别名

PP2C beta, PP2C beta X, PP2CB, PP2CBETA, PPC2BETAX, PPM1B, Protein phosphatase 1B

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman breast cancer tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67647-1-Ig targets PPM1B in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 PPM1B fusion protein Ag3871 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 protein phosphatase 1B (formerly 2C), magnesium-dependent, beta isoform
别名 PP2C beta, PP2C beta X, PP2CB, PP2CBETA, PPC2BETAX, PPM1B, Protein phosphatase 1B
计算分子量 479 aa, 53 kDa
观测分子量 36, 53 kDa
GenBank蛋白编号BC012002
基因名称 PPM1B
Gene ID (NCBI) 5495
RRIDAB_2882847
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO75688
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PPM1B can identify 4 isoforms with the WM of 36-55 kDa.

实验方案

Product Specific Protocols
WB protocol for PPM1B antibody 67647-1-IgDownload protocol
IHC protocol for PPM1B antibody 67647-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols