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PPM1B Monoclonal antibody

PPM1B Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2C7H2

Cat no : 67647-1-Ig

Print datasheet

Synonyms

PP2C beta, PP2C beta X, PP2CB, PP2CBETA, PPC2BETAX, PPM1B, Protein phosphatase 1B

验证数据展示

  • WB analysis using 67647-1-Ig

    WB analysis using 67647-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67647-1-Ig (PPM1B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis using 67647-1-Ig

    WB analysis using 67647-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67647-1-Ig (PPM1B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 67647-1-Ig

    IHC staining of human breast cancer using 67647-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67647-1-Ig (PPM1B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 67647-1-Ig

    IHC staining of human breast cancer using 67647-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67647-1-Ig (PPM1B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman breast cancer tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67647-1-Ig targets PPM1B in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen PPM1B fusion protein Ag3871 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name protein phosphatase 1B (formerly 2C), magnesium-dependent, beta isoform
Synonyms PP2C beta, PP2C beta X, PP2CB, PP2CBETA, PPC2BETAX, PPM1B, Protein phosphatase 1B
Calculated Molecular Weight 479 aa, 53 kDa
Observed Molecular Weight 36, 53 kDa
GenBank Accession NumberBC012002
Gene Symbol PPM1B
Gene ID (NCBI) 5495
RRIDAB_2882847
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO75688
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PPM1B can identify 4 isoforms with the WM of 36-55 kDa.

实验方案

Product Specific Protocols
WB protocol for PPM1B antibody 67647-1-IgDownload protocol
IHC protocol for PPM1B antibody 67647-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols