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PPM1B Monoclonal antibody, PBS Only

PPM1B Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2C7H2

Cat no : 67647-1-PBS

Print datasheet

Synonyms

PP2C beta, PP2C beta X, PP2CB, PP2CBETA, PPC2BETAX, PPM1B, Protein phosphatase 1B

验证数据展示

  • WB analysis using 67647-1-Ig (same clone as 67647-1-PBS)

    WB analysis using 67647-1-Ig (same clone as 67647-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67647-1-Ig (PPM1B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67647-1-PBS in a different storage buffer formulation.

  • WB analysis using 67647-1-Ig (same clone as 67647-1-PBS)

    WB analysis using 67647-1-Ig (same clone as 67647-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67647-1-Ig (PPM1B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67647-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67647-1-Ig (same clone as 67647-1-PBS)

    IHC staining of human breast cancer using 67647-1-Ig (same clone as 67647-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67647-1-Ig (PPM1B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67647-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67647-1-Ig (same clone as 67647-1-PBS)

    IHC staining of human breast cancer using 67647-1-Ig (same clone as 67647-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67647-1-Ig (PPM1B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67647-1-PBS in a different storage buffer formulation.

产品信息

67647-1-PBS targets PPM1B in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen PPM1B fusion protein Ag3871 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name protein phosphatase 1B (formerly 2C), magnesium-dependent, beta isoform
Synonyms PP2C beta, PP2C beta X, PP2CB, PP2CBETA, PPC2BETAX, PPM1B, Protein phosphatase 1B
Calculated Molecular Weight 479 aa, 53 kDa
Observed Molecular Weight 36, 53 kDa
GenBank Accession NumberBC012002
Gene Symbol PPM1B
Gene ID (NCBI) 5495
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO75688
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PPM1B can identify 4 isoforms with the WM of 36-55 kDa.