• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 25089-1-AP (same clone as 25089-1-PBS)

    WB analysis using 25089-1-AP (same clone as 25089-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25089-1-AP (PPM1E antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25089-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 25089-1-AP (same clone as 25089-1-PBS)

    IHC staining of rat brain using 25089-1-AP (same clone as 25089-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 25089-1-AP (PPM1E antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25089-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 25089-1-AP (same clone as 25089-1-PBS)

    IF Staining of A431 using 25089-1-AP (same clone as 25089-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using PPM1E antibody (25089-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 25089-1-PBS in a different storage buffer formulation.

  • IF Staining of SH-SY5Y using 25089-1-AP (same clone as 25089-1-PBS)

    IF Staining of SH-SY5Y using 25089-1-AP (same clone as 25089-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using PPM1E antibody (25089-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 25089-1-PBS in a different storage buffer formulation.

PPM1E Polyclonal antibody, PBS Only

PPM1E Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 25089-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

25089-1-PBS targets PPM1E in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PPM1E fusion protein Ag18751 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein phosphatase 1E (PP2C domain containing)
别名
计算分子量 764 aa, 85 kDa
观测分子量85 kDa
GenBank蛋白编号BC136290
基因名称 PPM1E
Gene ID (NCBI) 22843
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8WY54
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Ca2+/calmodulin-dependent protein kinase phosphatase (CaMKP-N),also known as Ppm1E, is an AMPKα phosphatase,which is up-regulated in human gastric cancer tissues (PMID: 28423719).