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PPM1M Polyclonal antibody

PPM1M Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 12104-1-AP

Print datasheet

Synonyms

PP2C eta, PP2CE, PP2Ceta, PPM1E, PPM1M, Protein phosphatase 1M

验证数据展示

  • WB analysis of human lung using 12104-1-AP

    WB analysis of human lung using 12104-1-AP

    human lung tissue were subjected to SDS PAGE followed by western blot with 12104-1-AP (PPM1M antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse lung using 12104-1-AP

    IP experiment of mouse lung using 12104-1-AP

    IP result of anti-PPM1M (IP:12104-1-AP, 4ug; Detection:12104-1-AP 1:300) with mouse lung tissue lysate 4000ug.

经过测试的应用

Positive WB detected inhuman lung tissue
Positive IP detected inmouse lung tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12104-1-AP targets PPM1M in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IP, ELISA Application Description
Tested Reactivity human, mouse
Immunogen PPM1M fusion protein Ag2748 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein phosphatase 1M (PP2C domain containing)
Synonyms PP2C eta, PP2CE, PP2Ceta, PPM1E, PPM1M, Protein phosphatase 1M
Calculated Molecular Weight 270 aa, 30 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC009644
Gene Symbol PPM1M
Gene ID (NCBI) 132160
RRIDAB_2170362
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96MI6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PPM1M antibody 12104-1-APDownload protocol
IP protocol for PPM1M antibody 12104-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols