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  • Featured Product
  • KD/KO Validated

PPP1CB-Specific Polyclonal antibody

PPP1CB-Specific Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 55136-1-AP

Print datasheet

Synonyms

PPP1CB, PPP1CD, PP-1B, PP1 Beta, PP 1B

验证数据展示

  • WB analysis using 55136-1-AP

    WB analysis using 55136-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 55136-1-AP (PPP1CB-Specific antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using 55136-1-AP

    IF Staining of A549 using 55136-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed A549 cells using 55136-1-AP (PPP1CB-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human skin cancer using 55136-1-AP

    IHC staining of human skin cancer using 55136-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 55136-1-AP (PPP1CB-Specific antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PPP1CB antibody included

经过测试的应用

Positive WB detected inA549 cells, BxPC-3 cells, NIH/3T3 cells, SH-SY5Y cells, mouse brain tissue
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells
Planning an IHC experiment? We recommend our IHCeasy PPP1CB Ready-To-Use IHC Kit. PPP1CB primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55136-1-AP targets PPP1CB-Specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein phosphatase 1, catalytic subunit, beta isoform
Synonyms PPP1CB, PPP1CD, PP-1B, PP1 Beta, PP 1B
Calculated Molecular Weight 37 kDa
Observed Molecular Weight 33-40 kDa
GenBank Accession NumberNM_002709
Gene Symbol PPP1CB
Gene ID (NCBI) 5500
RRIDAB_10837232
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP62140
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PPP1CB, also named as PP-1B, belongs to the PPP phosphatase family and PP-1 subfamily. Protein phosphatase (PP1) is essential for cell division, it participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. PPP1CB is involved in regulation of ionic conductances and long-term synaptic plasticity. This antibody is specific to PPP1CB.

实验方案

Product Specific Protocols
WB protocol for PPP1CB-Specific antibody 55136-1-APDownload protocol
IHC protocol for PPP1CB-Specific antibody 55136-1-APDownload protocol
IF protocol for PPP1CB-Specific antibody 55136-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

PLoS Pathog

Protein phosphatase 1 catalyzes HBV core protein dephosphorylation and is co-packaged with viral pregenomic RNA into nucleocapsids.

Authors - Zhanying Hu
  • KD Validated
View Article
humanWB

Exp Eye Res

PPP1CA/YAP/GS/Gln/mTORC1 pathway activates retinal Müller cells during diabetic retinopathy.

Authors - Yang Guo
View Article