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验证数据展示

  • WB analysis using 55136-1-AP

    WB analysis using 55136-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 55136-1-AP (PPP1CB-Specific antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using 55136-1-AP

    IF Staining of A549 using 55136-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed A549 cells using 55136-1-AP (PPP1CB-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human skin cancer using 55136-1-AP

    IHC staining of human skin cancer using 55136-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 55136-1-AP (PPP1CB-Specific antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PPP1CB antibody included
  • Featured Product
  • KD/KO验证

PPP1CB-Specific Polyclonal antibody

PPP1CB-Specific Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 55136-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

PPP1CB, PPP1CD, PP-1B, PP1 Beta, PP 1B

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, BxPC-3 cells, NIH/3T3 cells, SH-SY5Y cells, mouse brain tissue
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells
Planning an IHC experiment? We recommend our IHCeasy PPP1CB Ready-To-Use IHC Kit. PPP1CB primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55136-1-AP targets PPP1CB-Specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein phosphatase 1, catalytic subunit, beta isoform
别名 PPP1CB, PPP1CD, PP-1B, PP1 Beta, PP 1B
计算分子量 37 kDa
观测分子量 33-40 kDa
GenBank蛋白编号NM_002709
基因名称 PPP1CB
Gene ID (NCBI) 5500
RRIDAB_10837232
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP62140
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PPP1CB, also named as PP-1B, belongs to the PPP phosphatase family and PP-1 subfamily. Protein phosphatase (PP1) is essential for cell division, it participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. PPP1CB is involved in regulation of ionic conductances and long-term synaptic plasticity. This antibody is specific to PPP1CB.

实验方案

Product Specific Protocols
WB protocol for PPP1CB-Specific antibody 55136-1-APDownload protocol
IHC protocol for PPP1CB-Specific antibody 55136-1-APDownload protocol
IF protocol for PPP1CB-Specific antibody 55136-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

PLoS Pathog

Protein phosphatase 1 catalyzes HBV core protein dephosphorylation and is co-packaged with viral pregenomic RNA into nucleocapsids.

Authors - Zhanying Hu
  • KD Validated
View Article
humanWB

Exp Eye Res

PPP1CA/YAP/GS/Gln/mTORC1 pathway activates retinal Müller cells during diabetic retinopathy.

Authors - Yang Guo
View Article