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验证数据展示

  • FC experiment of U2OS using CL488-55136

    FC experiment of U2OS using CL488-55136

    1x10^6 U2OS cells were intracellularly stained with 0.8 ug Coralite® Plus 488 PPP1CB-Specific Polyclonal Antibody (CL488-55136)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of U2OS using CL488-55136

    IF Staining of U2OS using CL488-55136

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CoraLite® Plus 488 PPP1CB-Specific antibody (CL488-55136) at dilution of 1:200, CL594-Phalloidin (red).

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  • KD/KO验证

CoraLite® Plus 488-conjugated PPP1CB-Specific Polyclonal antibody

PPP1CB-Specific Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-55136

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

PPP1CB, PPP1CD, PP-1B, PP1 Beta, PP 1B

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inU2OS cells
Planning an IHC experiment? We recommend our IHCeasy PPP1CB Ready-To-Use IHC Kit. PPP1CB primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 55136-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-55136 targets PPP1CB-Specific in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein phosphatase 1, catalytic subunit, beta isoform
别名 PPP1CB, PPP1CD, PP-1B, PP1 Beta, PP 1B
计算分子量 37 kDa
观测分子量 33-40 kDa
GenBank蛋白编号NM_002709
基因名称 PPP1CB
Gene ID (NCBI) 5500
RRIDAB_3672855
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP62140
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PPP1CB, also named as PP-1B, belongs to the PPP phosphatase family and PP-1 subfamily. Protein phosphatase (PP1) is essential for cell division, it participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. PPP1CB is involved in regulation of ionic conductances and long-term synaptic plasticity. This antibody is specific to PPP1CB.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 PPP1CB-Specific antibody CL488-55136Download protocol
FC protocol for CL Plus 488 PPP1CB-Specific antibody CL488-55136Download protocol
Standard Protocols
Click here to view our Standard Protocols