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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated PPP1CB-Specific Polyclonal antibody

PPP1CB-Specific Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-55136

Print datasheet

Synonyms

PPP1CB, PPP1CD, PP-1B, PP1 Beta, PP 1B

验证数据展示

  • FC experiment of U2OS using CL488-55136

    FC experiment of U2OS using CL488-55136

    1x10^6 U2OS cells were intracellularly stained with 0.8 ug Coralite® Plus 488 PPP1CB-Specific Polyclonal Antibody (CL488-55136)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of U2OS using CL488-55136

    IF Staining of U2OS using CL488-55136

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CoraLite® Plus 488 PPP1CB-Specific antibody (CL488-55136) at dilution of 1:200, CL594-Phalloidin (red).

经过测试的应用

Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inU2OS cells
Planning an IHC experiment? We recommend our IHCeasy PPP1CB Ready-To-Use IHC Kit. PPP1CB primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 55136-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-55136 targets PPP1CB-Specific in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein phosphatase 1, catalytic subunit, beta isoform
Synonyms PPP1CB, PPP1CD, PP-1B, PP1 Beta, PP 1B
Calculated Molecular Weight 37 kDa
Observed Molecular Weight 33-40 kDa
GenBank Accession NumberNM_002709
Gene Symbol PPP1CB
Gene ID (NCBI) 5500
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP62140
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PPP1CB, also named as PP-1B, belongs to the PPP phosphatase family and PP-1 subfamily. Protein phosphatase (PP1) is essential for cell division, it participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. PPP1CB is involved in regulation of ionic conductances and long-term synaptic plasticity. This antibody is specific to PPP1CB.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 PPP1CB-Specific antibody CL488-55136Download protocol
FC protocol for CL Plus 488 PPP1CB-Specific antibody CL488-55136Download protocol
Standard Protocols
Click here to view our Standard Protocols