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CoraLite® Plus 488-conjugated PPP2CA Monoclonal antibody

PPP2CA Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3A6H5

Cat no : CL488-67809

Print datasheet

Synonyms

PP2CA, PP2Ac, PP2A-alpha, PP2A alpha, EC:3.1.3.16

验证数据展示

  • FC experiment of MCF-7 using CL488-67809

    FC experiment of MCF-7 using CL488-67809

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human PPP2CA (CL488-67809, Clone:3A6H5) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive FC (Intra) detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy PPP2CA Ready-To-Use IHC Kit. PPP2CA primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67809-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67809 targets PPP2CA in FC (Intra) applications and shows reactivity with human, rat samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, rat
Immunogen PPP2CA fusion protein Ag4294 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform
Synonyms PP2CA, PP2Ac, PP2A-alpha, PP2A alpha, EC:3.1.3.16
Calculated Molecular Weight 309 aa, 36 kDa
Observed Molecular Weight33 kDa
GenBank Accession NumberBC031696
Gene Symbol PPP2CA
Gene ID (NCBI) 5515
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP67775
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PPP2CA, also named as RP-C and PP2A-alpha, belongs to the PPP phosphatase family and PP-1 subfamily. PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. PP2A activity was modestly decreased in AML patients harboring C-KIT/WT.PP2A is a target for C-KIT/TKD and implicated the potential efficacy of therapies targeting PP2A in such AML in future.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 PPP2CA antibody CL488-67809Download protocol
Standard Protocols
Click here to view our Standard Protocols