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验证数据展示

  • FC experiment of MCF-7 using CL488-67809

    FC experiment of MCF-7 using CL488-67809

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human PPP2CA (CL488-67809, Clone:3A6H5) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 488-conjugated PPP2CA Monoclonal antibody

PPP2CA Monoclonal Antibody for FC (Intra)
Cat No. CL488-67809

产品说明书

CloneNo. 3A6H5

宿主/亚型

Mouse / IgG1

种属反应性

human, rat

应用

FC (Intra)

别名

PP2CA, PP2Ac, PP2A-alpha, PP2A alpha, EC:3.1.3.16

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy PPP2CA Ready-To-Use IHC Kit. PPP2CA primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67809-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67809 targets PPP2CA in FC (Intra) applications and shows reactivity with human, rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, rat
免疫原 PPP2CA fusion protein Ag4294 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform
别名 PP2CA, PP2Ac, PP2A-alpha, PP2A alpha, EC:3.1.3.16
计算分子量 309 aa, 36 kDa
观测分子量33 kDa
GenBank蛋白编号BC031696
基因名称 PPP2CA
Gene ID (NCBI) 5515
RRIDAB_2923794
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP67775
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PPP2CA, also named as RP-C and PP2A-alpha, belongs to the PPP phosphatase family and PP-1 subfamily. PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. PP2A activity was modestly decreased in AML patients harboring C-KIT/WT.PP2A is a target for C-KIT/TKD and implicated the potential efficacy of therapies targeting PP2A in such AML in future.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 PPP2CA antibody CL488-67809Download protocol
Standard Protocols
Click here to view our Standard Protocols