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PPP2R1A Polyclonal antibody
PPP2R1A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, IF/ICC, IP, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HepG2 cells, A431 cells, mouse kidney tissue, rat liver tissue, SMMC-7721 cells, mouse liver tissue, L02 cells |
Positive IP detected in | HepG2 cells |
Positive IHC detected in | mouse kidney tissue, human kidney tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HEK-293 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
KD/KO | See 3 publications below |
WB | See 7 publications below |
CoIP | See 2 publications below |
产品信息
15882-1-AP targets PPP2R1A in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
Cited Applications | WB, CoIP |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, pig |
Immunogen | PPP2R1A fusion protein Ag8661 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | protein phosphatase 2 (formerly 2A), regulatory subunit A, alpha isoform |
Synonyms | Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform, PR65A, PP2A subunit A isoform R1-alpha, PP2A subunit A isoform PR65-alpha, Medium tumor antigen-associated 61 kDa protein |
Calculated Molecular Weight | 589 aa, 65 kDa |
Observed Molecular Weight | 65 kDa |
GenBank Accession Number | BC001537 |
Gene Symbol | PPP2R1A |
Gene ID (NCBI) | 5518 |
RRID | AB_2237574 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P30153 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for PPP2R1A antibody 15882-1-AP | Download protocol |
IHC protocol for PPP2R1A antibody 15882-1-AP | Download protocol |
IF protocol for PPP2R1A antibody 15882-1-AP | Download protocol |
IP protocol for PPP2R1A antibody 15882-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Science Identification of Integrator-PP2A complex (INTAC), an RNA polymerase II phosphatase. | ||
Mol Cell SPT5 stabilizes RNA polymerase II, orchestrates transcription cycles, and maintains the enhancer landscape. | ||
Front Cell Dev Biol BRG1 Activates PR65A Transcription to Regulate NO Bioavailability in Vascular Endothelial Cells. | ||
Cancer Cell Int PPP2R1A silencing suppresses LUAD progression by sensitizing cells to nelfinavir-induced apoptosis and pyroptosis
| ||
Microbiol Spectr TurboID Screening of the OmpP2 Protein Reveals Host Proteins Involved in Recognition and Phagocytosis of Glaesserella parasuis by iPAM Cells
| ||
Dev Cell Fam72a functions as a cell-cycle-controlled gene during proliferation and antagonizes apoptosis through reprogramming PP2A substrates |