验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, A431 cells, mouse kidney tissue, rat liver tissue, SMMC-7721 cells, mouse liver tissue, L02 cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | mouse kidney tissue, human kidney tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
Planning an IHC experiment? We recommend our IHCeasy PPP2R1A Ready-To-Use IHC Kit. PPP2R1A primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 3 publications below |
| WB | See 7 publications below |
| CoIP | See 2 publications below |
产品信息
15882-1-AP targets PPP2R1A in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, pig |
| 免疫原 | PPP2R1A fusion protein Ag8661 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | protein phosphatase 2 (formerly 2A), regulatory subunit A, alpha isoform |
| 别名 | Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform, PR65A, PP2A subunit A isoform R1-alpha, PP2A subunit A isoform PR65-alpha, Medium tumor antigen-associated 61 kDa protein |
| 计算分子量 | 589 aa, 65 kDa |
| 观测分子量 | 65 kDa |
| GenBank蛋白编号 | BC001537 |
| 基因名称 | PPP2R1A |
| Gene ID (NCBI) | 5518 |
| RRID | AB_2237574 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P30153 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PPP2R1A belongs to the phosphatase 2A regulatory subunit A family. PPP2R1A encodes the α-isoform of the scaffolding subunit of the serine/threonine protein phosphatase 2A (PP2A) holoenzyme. This tumour suppressor complex is involved in growth and survival pathways and PPP2R1A mutations in endometrial high-grade serous carcinomas are a frequent and potentially targetable feature of this disease(PMID: 21381030). The PPP2R1A gene has 86% identity with the PPP2R1B gene(PMID:7681822).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PPP2R1A antibody 15882-1-AP | Download protocol |
| IHC protocol for PPP2R1A antibody 15882-1-AP | Download protocol |
| IF protocol for PPP2R1A antibody 15882-1-AP | Download protocol |
| IP protocol for PPP2R1A antibody 15882-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Science Identification of Integrator-PP2A complex (INTAC), an RNA polymerase II phosphatase. | ||
Mol Cell SPT5 stabilizes RNA polymerase II, orchestrates transcription cycles, and maintains the enhancer landscape. | ||
Front Cell Dev Biol BRG1 Activates PR65A Transcription to Regulate NO Bioavailability in Vascular Endothelial Cells. | ||
Cancer Cell Int PPP2R1A silencing suppresses LUAD progression by sensitizing cells to nelfinavir-induced apoptosis and pyroptosis
| ||
Microbiol Spectr TurboID Screening of the OmpP2 Protein Reveals Host Proteins Involved in Recognition and Phagocytosis of Glaesserella parasuis by iPAM Cells
| ||
Dev Cell Fam72a functions as a cell-cycle-controlled gene during proliferation and antagonizes apoptosis through reprogramming PP2A substrates |
