CoraLite® Plus 488-conjugated PPP2R2A/B/C Monoclonal antibody
PPP2R2A/B/C Monoclonal Antibody for FC (Inrta)
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse, Rat, Pig, Rabbit
Applications
FC (Inrta)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
2E1D5
验证数据展示
经过测试的应用
| Positive FC (Inrta) detected in | HeLa cells |
For other applications, we recommend the unconjugated version of this antibody, 67783-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) (INRTA) | FC (INRTA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-67783 targets PPP2R2A/B/C in FC (Inrta) applications and shows reactivity with Human, Mouse, Rat, Pig, Rabbit samples.
| Tested Applications | FC (Inrta) Application Description |
| Tested Reactivity | Human, Mouse, Rat, Pig, Rabbit |
| Immunogen | PPP2R2A/B/C fusion protein Ag30774 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | protein phosphatase 2 (formerly 2A), regulatory subunit B, beta isoform |
| Synonyms | PPP2R2B, PP2A subunit B isoform B55-beta, PP2A PR55B, PP2A B55BETA, B55β |
| Calculated Molecular Weight | 443 aa, 52 kDa |
| Observed Molecular Weight | 52 kDa |
| GenBank Accession Number | BC031790 |
| Gene Symbol | PPP2R2B |
| Gene ID (NCBI) | 5521 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q00005 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The PPP2R2B gene encodes a deduced 443-amino acid protein of approximately 52 kDa,which is a brain-specific regulatory subunit B of protein phosphatase 2. PPP2R2B is a Subunit of PP2A, a highly conserved constitutive enzyme(PMID:11719278). PPP2R2B (Bβ) is an important regulator of protein phosphatase 2A (PP2A) activity in the brain. Through differential promoter usage and alternative splicing, two major isoforms Bβ1 and Bβ2 with divergent sub-cellular targeting N termini are produced. Bβ plays an important role in neuronal survival. It has 5 isoforms produced by alternative splicing.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 PPP2R2A/B/C antibody CL488-67783 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
