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验证数据展示

  • WB analysis of human ileum using 12675-2-AP (same clone as 12675-2-PBS)

    WB analysis of human ileum using 12675-2-AP (same clone as 12675-2-PBS)

    human ileum tissue were subjected to SDS PAGE followed by western blot with 12675-2-AP (PPP2R5A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12675-2-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 12675-2-AP (same clone as 12675-2-PBS)

    WB analysis of HeLa using 12675-2-AP (same clone as 12675-2-PBS)

    WB result of PPP2R5A antibody (12675-2-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-PPP2R5A transfected HeLa cells. This data was developed using the same antibody clone with 12675-2-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 12675-2-AP (same clone as 12675-2-PBS)

    IP experiment of mouse brain using 12675-2-AP (same clone as 12675-2-PBS)

    IP result of anti-PPP2R5A (IP:12675-2-AP, 4ug; Detection:12675-2-AP 1:500) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 12675-2-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 12675-2-AP (same clone as 12675-2-PBS)

    IHC staining of human pancreas cancer using 12675-2-AP (same clone as 12675-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12675-2-AP (PPP2R5A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12675-2-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 12675-2-AP (same clone as 12675-2-PBS)

    IHC staining of human pancreas cancer using 12675-2-AP (same clone as 12675-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12675-2-AP (PPP2R5A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12675-2-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 12675-2-AP (same clone as 12675-2-PBS)

    IF Staining of HepG2 using 12675-2-AP (same clone as 12675-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 12675-2-AP (PPP2R5A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12675-2-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

PPP2R5A Polyclonal antibody, PBS Only

PPP2R5A Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 12675-2-PBS

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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产品信息

12675-2-PBS targets PPP2R5A in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen PPP2R5A fusion protein Ag3360 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein phosphatase 2, regulatory subunit B', alpha isoform
Synonyms
Calculated Molecular Weight 486 aa, 56 kDa
Observed Molecular Weight 56 kDa
GenBank Accession NumberBC022474
Gene Symbol PPP2R5A
Gene ID (NCBI) 5525
RRIDAB_2252956
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15172
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PPP2R5A(protein phosphatase 2, regulatory subunit B (B56), alpha isoform) is also named as PR61alpha and belongs to the phosphatase 2A regulatory subunit B56 family. B56 alpha subunit gene encodes a cytoplasmic phosphoprotein(PMID:7592815). The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. This protein has 2 isoforms produced by alternative splicing with the molecular weight of 54 kDa and 50 kDa.