验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HeLa cells, A375 cells, mouse testis tissue, K-562 cells, U2OS cells |
| Positive IF/ICC detected in | HaCaT cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 1 publications below |
产品信息
68097-1-Ig targets PRAME in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | IHC |
| 经测试反应性 | Human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | PRAME fusion protein Ag1906 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | preferentially expressed antigen in melanoma |
| 别名 | MAPE, OIP 4, OIP4, Opa interacting protein 4, PRAME |
| 计算分子量 | 509 aa, 58 kDa |
| 观测分子量 | 50 kDa |
| GenBank蛋白编号 | BC014074 |
| 基因名称 | PRAME |
| Gene ID (NCBI) | 23532 |
| RRID | AB_2918834 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P78395 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The PRAME (preferentially expressed antigen of melanoma) gene was previously shown to be overexpressed in ovarian/primary peritoneal serous carcinoma compared with malignant mesothelioma using gene expression arrays. It is considered a melanocyte differentiation antigen which is overexpressed in both solid and hematologic tumors. In normal tissue, a very low level of PRAME expression is found in normal testis, adrenals, ovary and endometrium. A high level of PRAME expression has been reported for several solid tumors, including ovarian cancer, breast cancer, lung cancer and melanomas, medulloblastoma, sarcomas, head and neck cancers, neuroblastoma, renal cancer, and Wilms'tumor. As a nuclear transcriptional repressor protein, PRAME binds to retinoic acid receptor a, thereby inhibiting retinoic acid induced differentiation, growth arrest, and apoptosis.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PRAME antibody 68097-1-Ig | Download protocol |
| IF protocol for PRAME antibody 68097-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
