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  • Featured Product
  • KD/KO Validated

PRAME Monoclonal antibody, PBS Only

PRAME Monoclonal Antibody for WB,Indirect ELISA,IF

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB,Indirect ELISA,IF

Conjugate

Unconjugated

CloneNo.

1E9G9

Cat No : 68097-1-PBS

Print datasheet

Synonyms

MAPE, OIP 4, OIP4, Opa interacting protein 4, PRAME

验证数据展示

  • WB analysis using 68097-1-Ig (same clone as 68097-1-PBS)

    WB analysis using 68097-1-Ig (same clone as 68097-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68097-1-Ig (PRAME antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68097-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 68097-1-Ig (same clone as 68097-1-PBS)

    WB analysis of HeLa using 68097-1-Ig (same clone as 68097-1-PBS)

    WB result of PRAME antibody (68097-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRAME transfected HeLa cells. This data was developed using the same antibody clone with 68097-1-PBS in a different storage buffer formulation.

  • IF Staining of HaCaT using 68097-1-Ig (same clone as 68097-1-PBS)

    IF Staining of HaCaT using 68097-1-Ig (same clone as 68097-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HaCaT cells using PRAME antibody (68097-1-Ig, Clone: 1E9G9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 68097-1-PBS in a different storage buffer formulation.

产品信息

68097-1-PBS targets PRAME in WB, IF, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB,Indirect ELISA,IF Application Description
Tested Reactivity Human, mouse, rat
Immunogen PRAME fusion protein Ag1906 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name preferentially expressed antigen in melanoma
Synonyms MAPE, OIP 4, OIP4, Opa interacting protein 4, PRAME
Calculated Molecular Weight 509 aa, 58 kDa
Observed Molecular Weight50 kDa
GenBank Accession NumberBC014074
Gene Symbol PRAME
Gene ID (NCBI) 23532
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP78395
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

The PRAME (preferentially expressed antigen of melanoma) gene was previously shown to be overexpressed in ovarian/primary peritoneal serous carcinoma compared with malignant mesothelioma using gene expression arrays. It is considered a melanocyte differentiation antigen which is overexpressed in both solid and hematologic tumors. In normal tissue, a very low level of PRAME expression is found in normal testis, adrenals, ovary and endometrium. A high level of PRAME expression has been reported for several solid tumors, including ovarian cancer, breast cancer, lung cancer and melanomas, medulloblastoma, sarcomas, head and neck cancers, neuroblastoma, renal cancer, and Wilms'tumor. As a nuclear transcriptional repressor protein, PRAME binds to retinoic acid receptor a, thereby inhibiting retinoic acid induced differentiation, growth arrest, and apoptosis.