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验证数据展示

  • WB analysis using 68097-1-Ig (same clone as 68097-1-PBS)

    WB analysis using 68097-1-Ig (same clone as 68097-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68097-1-Ig (PRAME antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68097-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 68097-1-Ig (same clone as 68097-1-PBS)

    WB analysis of HeLa using 68097-1-Ig (same clone as 68097-1-PBS)

    WB result of PRAME antibody (68097-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRAME transfected HeLa cells. This data was developed using the same antibody clone with 68097-1-PBS in a different storage buffer formulation.

  • IF Staining of HaCaT using 68097-1-Ig (same clone as 68097-1-PBS)

    IF Staining of HaCaT using 68097-1-Ig (same clone as 68097-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HaCaT cells using PRAME antibody (68097-1-Ig, Clone: 1E9G9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 68097-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

PRAME Monoclonal antibody, PBS Only

PRAME Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68097-1-PBS

产品说明书

CloneNo. 1E9G9

宿主/亚型

Mouse / IgG2b

种属反应性

Human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

MAPE, OIP 4, OIP4, Opa interacting protein 4, PRAME

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

68097-1-PBS targets PRAME in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 PRAME fusion protein Ag1906 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 preferentially expressed antigen in melanoma
别名 MAPE, OIP 4, OIP4, Opa interacting protein 4, PRAME
计算分子量 509 aa, 58 kDa
观测分子量50 kDa
GenBank蛋白编号BC014074
基因名称 PRAME
Gene ID (NCBI) 23532
RRIDAB_2918834
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP78395
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The PRAME (preferentially expressed antigen of melanoma) gene was previously shown to be overexpressed in ovarian/primary peritoneal serous carcinoma compared with malignant mesothelioma using gene expression arrays. It is considered a melanocyte differentiation antigen which is overexpressed in both solid and hematologic tumors. In normal tissue, a very low level of PRAME expression is found in normal testis, adrenals, ovary and endometrium. A high level of PRAME expression has been reported for several solid tumors, including ovarian cancer, breast cancer, lung cancer and melanomas, medulloblastoma, sarcomas, head and neck cancers, neuroblastoma, renal cancer, and Wilms'tumor. As a nuclear transcriptional repressor protein, PRAME binds to retinoic acid receptor a, thereby inhibiting retinoic acid induced differentiation, growth arrest, and apoptosis.