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验证数据展示

  • WB analysis using 67027-1-Ig (same clone as 67027-1-PBS)

    WB analysis using 67027-1-Ig (same clone as 67027-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67027-1-Ig (PRC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67027-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67027-1-Ig (same clone as 67027-1-PBS)

    IHC staining of human breast cancer using 67027-1-Ig (same clone as 67027-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67027-1-Ig (PRC1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67027-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67027-1-Ig (same clone as 67027-1-PBS)

    IHC staining of human breast cancer using 67027-1-Ig (same clone as 67027-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67027-1-Ig (PRC1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67027-1-PBS in a different storage buffer formulation.

  • WB analysis of MCF-7 using 67027-1-Ig (same clone as 67027-1-PBS)

    WB analysis of MCF-7 using 67027-1-Ig (same clone as 67027-1-PBS)

    WB result of PRC1 antibody (67027-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRC1 transfected MCF-7 cells. This data was developed using the same antibody clone with 67027-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 67027-1-Ig (same clone as 67027-1-PBS)

    IF Staining of HeLa using 67027-1-Ig (same clone as 67027-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PRC1 antibody (67027-1-Ig, Clone: 1E1D5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67027-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

PRC1 Monoclonal antibody, PBS Only

PRC1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67027-1-PBS

产品说明书

CloneNo. 1E1D5

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

ASE1, PRC1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

67027-1-PBS targets PRC1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 PRC1 fusion protein Ag5907 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 protein regulator of cytokinesis 1
别名 ASE1, PRC1
计算分子量 72 kDa
观测分子量 66-72 kDa
GenBank蛋白编号BC003138
基因名称 PRC1
Gene ID (NCBI) 9055
RRIDAB_2882342
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO43663
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PRC1 (protein regulator of cytokinesis 1) is a microtubule-bundling protein that is involved in cytokinesis. Depletion of PRC1 leads to failed cytokinesis. PRC1 is located in the nucleus during interphase, and becomes associated with mitotic spindles in a highly dynamic manner during mitosis, and localizes to the cell mid-body during cytokinesis. PRC1 is a substrate of CDK1 and it was assumed that CDK1 phosphorylation blocks its activity during metaphase. Three isoforms of PRC1 exist due to the alternative splicing, with predictive molecular weight of 71.6 kDa, 70.2 kDa and 66.2 kDa, respectively. The dual bands between 66-72 kDa detected by this antibody may represent different forms of PRC1.