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验证数据展示

  • WB analysis using 67027-1-Ig

    WB analysis using 67027-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67027-1-Ig (PRC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 67027-1-Ig

    IHC staining of human breast cancer using 67027-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67027-1-Ig (PRC1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 67027-1-Ig

    IHC staining of human breast cancer using 67027-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67027-1-Ig (PRC1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of MCF-7 using 67027-1-Ig

    WB analysis of MCF-7 using 67027-1-Ig

    WB result of PRC1 antibody (67027-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRC1 transfected MCF-7 cells.

  • IF Staining of HeLa using 67027-1-Ig

    IF Staining of HeLa using 67027-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PRC1 antibody (67027-1-Ig, Clone: 1E1D5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Featured Product
  • KD/KO验证

PRC1 Monoclonal antibody

PRC1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67027-1-Ig

产品说明书

CloneNo. 1E1D5

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

ASE1, PRC1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells, HEK-293 cells, A549 cells, HepG2 cells, HSC-T6 cells, 4T1 cells
Positive IHC detected inhuman breast cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67027-1-Ig targets PRC1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 PRC1 fusion protein Ag5907 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 protein regulator of cytokinesis 1
别名 ASE1, PRC1
计算分子量 72 kDa
观测分子量 66-72 kDa
GenBank蛋白编号BC003138
基因名称 PRC1
Gene ID (NCBI) 9055
RRIDAB_2882342
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO43663
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PRC1 (protein regulator of cytokinesis 1) is a microtubule-bundling protein that is involved in cytokinesis. Depletion of PRC1 leads to failed cytokinesis. PRC1 is located in the nucleus during interphase, and becomes associated with mitotic spindles in a highly dynamic manner during mitosis, and localizes to the cell mid-body during cytokinesis. PRC1 is a substrate of CDK1 and it was assumed that CDK1 phosphorylation blocks its activity during metaphase. Three isoforms of PRC1 exist due to the alternative splicing, with predictive molecular weight of 71.6 kDa, 70.2 kDa and 66.2 kDa, respectively. The dual bands between 66-72 kDa detected by this antibody may represent different forms of PRC1.

实验方案

Product Specific Protocols
WB protocol for PRC1 antibody 67027-1-IgDownload protocol
IHC protocol for PRC1 antibody 67027-1-IgDownload protocol
IF protocol for PRC1 antibody 67027-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols