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验证数据展示

  • IF Staining of HeLa using CL488-67027

    IF Staining of HeLa using CL488-67027

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 PRC1 antibody (CL488-67027, Clone: 1E1D5 ) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated PRC1 Monoclonal antibody

PRC1 Monoclonal Antibody for IF/ICC
Cat No. CL488-67027

产品说明书

CloneNo. 1E1D5

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat

应用

IF/ICC

别名

ASE1, PRC1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67027-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67027 targets PRC1 in IF/ICC applications and shows reactivity with Human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 Human, mouse, rat
免疫原 PRC1 fusion protein Ag5907 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 protein regulator of cytokinesis 1
别名 ASE1, PRC1
计算分子量 72 kDa
观测分子量 66-72 kDa
GenBank蛋白编号BC003138
基因名称 PRC1
Gene ID (NCBI) 9055
RRIDAB_2919409
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO43663
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PRC1 (protein regulator of cytokinesis 1) is a microtubule-bundling protein that is involved in cytokinesis. Depletion of PRC1 leads to failed cytokinesis. PRC1 is located in the nucleus during interphase, and becomes associated with mitotic spindles in a highly dynamic manner during mitosis, and localizes to the cell mid-body during cytokinesis. PRC1 is a substrate of CDK1 and it was assumed that CDK1 phosphorylation blocks its activity during metaphase. Three isoforms of PRC1 exist due to the alternative splicing, with predictive molecular weight of 71.6 kDa, 70.2 kDa and 66.2 kDa, respectively. The dual bands between 66-72 kDa detected by this antibody may represent different forms of PRC1.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 PRC1 antibody CL488-67027Download protocol
Standard Protocols
Click here to view our Standard Protocols