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  • Featured Product
  • KD/KO Validated

PRDX6 Monoclonal antibody

PRDX6 Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

3C12D3

Cat no : 67499-1-Ig

Print datasheet

Synonyms

EC:1.11.1.27, AOP2, Acidic calcium-independent phospholipase A2, 3C12D3, 1-Cys PRX

验证数据展示

  • WB analysis using 67499-1-Ig

    WB analysis using 67499-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67499-1-Ig (PRDX6 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis of HAP1 and HeLa using 67499-1-Ig

    WB analysis of HAP1 and HeLa using 67499-1-Ig

    HAP1 and HeLa (WT and PRDX6 KO) lysates prepared with RIPA buffer, 25ug protein loaded. 67499-1-Ig incubated at 1:1000 at 4°C overnight in 5% BSA in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

  • WB analysis of HeLa using 67499-1-Ig

    WB analysis of HeLa using 67499-1-Ig

    WB result of PRDX6 antibody (67499-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRDX6 transfected HeLa cells.

  • IP experiment of HAP1 using 67499-1-Ig

    IP experiment of HAP1 using 67499-1-Ig

    HAP1 lysates prepared and IP of peroxidoxin 6 performed using 2.0 μg of 67499-1-Ig coupled to protein G- Sepharose beads. Ponceau stained transfers shown for each blot. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

  • IF Staining of HAP1 using 67499-1-Ig

    IF Staining of HAP1 using 67499-1-Ig

    HAP1 WT cells (yellow outline) and PRDX6 KO cells (red outline) labelled with a green or a far red fluorescence dye, respectively. Cells fixed with 4% PFA and stained with 67499-1-lg at 1:1000 plus DAPI. Bars = 10 μm. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

  • IHC staining of human liver using 67499-1-Ig

    IHC staining of human liver using 67499-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67499-1-Ig (PRDX6 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PRDX6 antibody included
  • IHC staining of human liver using 67499-1-Ig

    IHC staining of human liver using 67499-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67499-1-Ig (PRDX6 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PRDX6 antibody included
  • IF Staining of HeLa using 67499-1-Ig

    IF Staining of HeLa using 67499-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PRDX6 antibody (67499-1-Ig, Clone: 3C12D3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inLNCaP cells, HAP1 cells, A549 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, pig brain tissue, HepG2 cells
Positive IP detected inHAP1 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, HAP1
Planning an IHC experiment? We recommend our IHCeasy PRDX6 Ready-To-Use IHC Kit. PRDX6 primary antibody included.
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67499-1-Ig targets PRDX6 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, pig samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Tested Reactivity human, pig
Immunogen PRDX6 fusion protein Ag4727 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name peroxiredoxin 6
Synonyms EC:1.11.1.27, AOP2, Acidic calcium-independent phospholipase A2, 3C12D3, 1-Cys PRX
Calculated Molecular Weight 224 aa, 25 kDa
Observed Molecular Weight 25-30 kDa
GenBank Accession NumberBC035857
Gene Symbol PRDX6
Gene ID (NCBI) 9588
RRIDAB_2882723
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP30041
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PRDX6 (Peroxiredoxin-6), also named as AOP2 or KIAA0106, is a unique member of the peroxiredoxin family of antioxidants. PRDX6 is highly expressed in liver and protects cells from oxidative damage by reducing H2O2 and various lipid Peroxides (PMID: 17382207). It can form a dimer(PMID:20500660).PRDX6 is expressed in all major organs, with a particularly high level in lung (PMID:15890616). Prdx6 is detected at approximately 24 to 28 kDa, and can be monosumoylated with the molecular mass of about 40 kDa (PMID: 24910119).

实验方案

Product Specific Protocols
WB protocol for PRDX6 antibody 67499-1-IgDownload protocol
IHC protocol for PRDX6 antibody 67499-1-IgDownload protocol
IF protocol for PRDX6 antibody 67499-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols