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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated PRDX6 Monoclonal antibody

PRDX6 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3C12D3

Cat No : CL594-67499

Print datasheet

Synonyms

PRDX6, 1-Cys peroxiredoxin, 1-Cys PRX, 3C12D3, Acidic calcium-independent phospholipase A2, AOP2, EC:1.11.1.27, EC:2.3.1.23, EC:3.1.1.4, Glutathione-dependent peroxiredoxin, KIAA0106, LPC acyltransferase 5, LPCAT-5, Lyso-PC acyltransferase 5, Lysophosphatidylcholine acyltransferase 5, Non-selenium glutathione peroxidase, peroxiredoxin 6, Peroxiredoxin-6

验证数据展示

  • FC experiment of HeLa using CL594-67499

    FC experiment of HeLa using CL594-67499

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human PRDX6 (CL594-67499, Clone:3C12D3) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL594-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PRDX6 Ready-To-Use IHC Kit. PRDX6 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67499-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67499 targets PRDX6 in FC (Intra) applications and shows reactivity with Human samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity Human
Immunogen PRDX6 fusion protein Ag4727 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name peroxiredoxin 6
Synonyms PRDX6, 1-Cys peroxiredoxin, 1-Cys PRX, 3C12D3, Acidic calcium-independent phospholipase A2, AOP2, EC:1.11.1.27, EC:2.3.1.23, EC:3.1.1.4, Glutathione-dependent peroxiredoxin, KIAA0106, LPC acyltransferase 5, LPCAT-5, Lyso-PC acyltransferase 5, Lysophosphatidylcholine acyltransferase 5, Non-selenium glutathione peroxidase, peroxiredoxin 6, Peroxiredoxin-6
Calculated Molecular Weight 224 aa, 25 kDa
Observed Molecular Weight 25-30 kDa
GenBank Accession NumberBC035857
Gene Symbol PRDX6
Gene ID (NCBI) 9588
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP30041
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PRDX6 (Peroxiredoxin-6), also named as AOP2 or KIAA0106, is a unique member of the peroxiredoxin family of antioxidants. PRDX6 is highly expressed in liver and protects cells from oxidative damage by reducing H2O2 and various lipid Peroxides (PMID: 17382207). It can form a dimer(PMID:20500660).PRDX6 is expressed in all major organs, with a particularly high level in lung (PMID:15890616). Prdx6 is detected at approximately 24 to 28 kDa, and can be monosumoylated with the molecular mass of about 40 kDa (PMID: 24910119).

实验方案

Product Specific Protocols
FC protocol for CL594 PRDX6 antibody CL594-67499Download protocol
Standard Protocols
Click here to view our Standard Protocols