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验证数据展示

  • FC experiment of HeLa using CL594-67499

    FC experiment of HeLa using CL594-67499

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human PRDX6 (CL594-67499, Clone:3C12D3) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL594-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of A431 using CL594-67499

    IF Staining of A431 using CL594-67499

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CoraLite®594 PRDX6 antibody (CL594-67499, Clone: 3C12D3 ) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite®594-conjugated PRDX6 Monoclonal antibody

PRDX6 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-67499

产品说明书

CloneNo. 3C12D3

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

EC:1.11.1.27, AOP2, Acidic calcium-independent phospholipase A2, 3C12D3, 1-Cys PRX

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PRDX6 Ready-To-Use IHC Kit. PRDX6 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67499-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67499 targets PRDX6 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原 PRDX6 fusion protein Ag4727 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 peroxiredoxin 6
别名 EC:1.11.1.27, AOP2, Acidic calcium-independent phospholipase A2, 3C12D3, 1-Cys PRX
计算分子量 224 aa, 25 kDa
观测分子量 25-30 kDa
GenBank蛋白编号BC035857
基因名称 PRDX6
Gene ID (NCBI) 9588
RRIDAB_2934769
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP30041
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PRDX6 (Peroxiredoxin-6), also named as AOP2 or KIAA0106, is a unique member of the peroxiredoxin family of antioxidants. PRDX6 is highly expressed in liver and protects cells from oxidative damage by reducing H2O2 and various lipid Peroxides (PMID: 17382207). It can form a dimer(PMID:20500660).PRDX6 is expressed in all major organs, with a particularly high level in lung (PMID:15890616). Prdx6 is detected at approximately 24 to 28 kDa, and can be monosumoylated with the molecular mass of about 40 kDa (PMID: 24910119).

实验方案

Product Specific Protocols
IF protocol for CL594 PRDX6 antibody CL594-67499Download protocol
Standard Protocols
Click here to view our Standard Protocols