验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, Neuro-2a cells |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse cerebellum tissue |
| Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
22589-1-AP targets PRICKLE1 in WB, IHC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF-P, FC (Intra), IP, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, chicken |
| 免疫原 | PRICKLE1 fusion protein Ag18407 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | prickle homolog 1 (Drosophila) |
| 别名 | RILP, REST/NRSF-interacting LIM domain protein 1, Prickle-like protein 1, EPM1B |
| 计算分子量 | 831 aa, 94 kDa |
| 观测分子量 | 94 kDa, 55-60 kDa |
| GenBank蛋白编号 | BC114939 |
| 基因名称 | PRICKLE1 |
| Gene ID (NCBI) | 144165 |
| RRID | AB_2879129 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen Affinity purified |
| UNIPROT ID | Q96MT3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PRICKLE1, also known as RILP, EPM1B, encodes a cytoplasmic protein with an N-terminal PET (Pk, Espinas, Testin) domain, three LIM domains and a C-terminal PKH (prickle homologous) domain. PRICKLE1 is expressed at the highest levels in placenta and lower levels in lung, liver, kidney and pancreas(PMID: 12525887). PRICKLE1 localizes to the nuclear membrane and has been implicated in the nuclear trafficking of the transcription repressors REST/NRSF and REST4. Mutations in PRICKLE1 have been linked to progressive myoclonus epilepsy(PMID: 18976727).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PRICKLE1 antibody 22589-1-AP | Download protocol |
| IHC protocol for PRICKLE1 antibody 22589-1-AP | Download protocol |
| IF protocol for PRICKLE1 antibody 22589-1-AP | Download protocol |
| IP protocol for PRICKLE1 antibody 22589-1-AP | Download protocol |
| FC protocol for PRICKLE1 antibody 22589-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Adv Prickle promotes the formation and maintenance of glutamatergic synapses by stabilizing the intercellular planar cell polarity complex.
| ||
Development Coupling of apical-basal polarity and PCP to interpret the Wnt signaling gradient and orient feather branch. | ||
Glia The formation of paranodal spirals at the ends of CNS myelin sheaths requires the planar polarity protein Vangl2. | ||
Exp Neurol Mutation of the murine Prickle1 (R104Q) causes phenotypes analogous to human symptoms of epilepsy and autism. | ||
J Transl Med PRICKLE1, a Wnt/PCP signaling component, is overexpressed and associated with inferior prognosis in acute myeloid leukemia. | ||
bioRxiv Differential Sensitivity of Midline Patterning to Mitosis during and after Primitive Streak Extension |
