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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated PRICKLE1 Polyclonal antibody

PRICKLE1 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-22589

Print datasheet

Synonyms

EPM1B, prickle homolog 1 (Drosophila), Prickle like protein 1, PRICKLE1, RILP

验证数据展示

  • FC experiment of HeLa using CL488-22589

    FC experiment of HeLa using CL488-22589

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human PRICKLE1 (CL488-22589)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PRICKLE1 Ready-To-Use IHC Kit. PRICKLE1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 22589-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-22589 targets PRICKLE1 in FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, mouse
Immunogen PRICKLE1 fusion protein Ag18407 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name prickle homolog 1 (Drosophila)
Synonyms EPM1B, prickle homolog 1 (Drosophila), Prickle like protein 1, PRICKLE1, RILP
Calculated Molecular Weight 831 aa, 94 kDa
Observed Molecular Weight 94 kDa, 55-60 kDa
GenBank Accession NumberBC114939
Gene Symbol PRICKLE1
Gene ID (NCBI) 144165
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96MT3
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PRICKLE1, also known as RILP, EPM1B, encodes a cytoplasmic protein with an N-terminal PET (Pk, Espinas, Testin) domain, three LIM domains and a C-terminal PKH (prickle homologous) domain. PRICKLE1 is expressed at the highest levels in placenta and lower levels in lung, liver, kidney and pancreas(PMID: 12525887). PRICKLE1 localizes to the nuclear membrane and has been implicated in the nuclear trafficking of the transcription repressors REST/NRSF and REST4. Mutations in PRICKLE1 have been linked to progressive myoclonus epilepsy(PMID: 18976727).

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 PRICKLE1 antibody CL488-22589Download protocol
Standard Protocols
Click here to view our Standard Protocols