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AMPK Beta 2 Polyclonal antibody

AMPK Beta 2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14429-1-AP

Print datasheet

Synonyms

PRKAB2, AMPK subunit beta-2, AMPK subunit beta 2, AMPK, 5'-AMP-activated protein kinase subunit beta-2

验证数据展示

  • WB analysis using 14429-1-AP

    WB analysis using 14429-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14429-1-AP (AMPK beta 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 14429-1-AP

    IHC staining of human colon using 14429-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14429-1-AP (AMPK Beta 2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PRKAB2 antibody included
  • IHC staining of human colon using 14429-1-AP

    IHC staining of human colon using 14429-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14429-1-AP (AMPK Beta 2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PRKAB2 antibody included
  • IF Staining of HeLa using 14429-1-AP

    IF Staining of HeLa using 14429-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using AMPK Beta 2 antibody (14429-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inHEK-293 cells, human heart tissue, human brain tissue, fetal human brain tissue, mouse testis tissue, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman colon tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PRKAB2 Ready-To-Use IHC Kit. PRKAB2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14429-1-AP targets AMPK Beta 2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen AMPK Beta 2 fusion protein Ag5806 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein kinase, AMP-activated, beta 2 non-catalytic subunit
Synonyms PRKAB2, AMPK subunit beta-2, AMPK subunit beta 2, AMPK, 5'-AMP-activated protein kinase subunit beta-2
Calculated Molecular Weight 30 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC053610
Gene Symbol AMPK Beta 2
Gene ID (NCBI) 5565
RRIDAB_2252925
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43741
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for AMPK Beta 2 antibody 14429-1-APDownload protocol
IHC protocol for AMPK Beta 2 antibody 14429-1-APDownload protocol
IF protocol for AMPK Beta 2 antibody 14429-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols