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验证数据展示

  • IF Staining of HeLa using CL488-12232

    IF Staining of HeLa using CL488-12232

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 PRKACB antibody (CL488-12232) at dilution of 1:200.

CoraLite® Plus 488-conjugated PRKACB Polyclonal antibody

PRKACB Polyclonal Antibody for IF/ICC
Cat No. CL488-12232

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Synonyms

DKFZp781I2452, PKA C beta, PKACB, PRKACB

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PRKACB Ready-To-Use IHC Kit. PRKACB primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 12232-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-12232 targets PRKACB in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, rat
Immunogen PRKACB fusion protein Ag2870 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein kinase, cAMP-dependent, catalytic, beta
Synonyms DKFZp781I2452, PKA C beta, PKACB, PRKACB
Calculated Molecular Weight 41 kDa, 46 kDa
Observed Molecular Weight 36-55 kDa
GenBank Accession NumberBC016285
Gene Symbol PRKACB
Gene ID (NCBI) 5567
RRIDAB_3672529
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP22694
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PRKACB, also named as PKA C-beta, belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family and cAMP subfamily. It mediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 PRKACB antibody CL488-12232Download protocol
Standard Protocols
Click here to view our Standard Protocols