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PRKAR2A Monoclonal antibody, PBS Only

PRKAR2A Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B2B3

Cat no : 67751-1-PBS

Print datasheet

Synonyms

PKR2, PRKAR2, PRKAR2A

验证数据展示

  • WB analysis using 67751-1-Ig (same clone as 67751-1-PBS)

    WB analysis using 67751-1-Ig (same clone as 67751-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67751-1-Ig (PRKAR2A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67751-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 67751-1-Ig (same clone as 67751-1-PBS)

    IHC staining of human colon cancer using 67751-1-Ig (same clone as 67751-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67751-1-Ig (PRKAR2A antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67751-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PRKAR2A antibody included
  • IHC staining of human colon cancer using 67751-1-Ig (same clone as 67751-1-PBS)

    IHC staining of human colon cancer using 67751-1-Ig (same clone as 67751-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67751-1-Ig (PRKAR2A antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67751-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PRKAR2A antibody included
  • IF Staining of HeLa using 67751-1-Ig (same clone as 67751-1-PBS)

    IF Staining of HeLa using 67751-1-Ig (same clone as 67751-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PRKAR2A antibody (67751-1-Ig, Clone: 1B2B3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67751-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy PRKAR2A Ready-To-Use IHC Kit. PRKAR2A primary antibody included.

产品信息

67751-1-PBS targets PRKAR2A in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, rat, mouse
Immunogen PRKAR2A fusion protein Ag29893 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name protein kinase, cAMP-dependent, regulatory, type II, alpha
Synonyms PKR2, PRKAR2, PRKAR2A
Calculated Molecular Weight 43 kDa
Observed Molecular Weight50 kDa
GenBank Accession NumberBC002763
Gene Symbol PRKAR2A
Gene ID (NCBI) 5576
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP13861
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The effects of cAMP in most tissues and cell types are mainly modulated via protein kinase A, a heterotetrameric protein complex consisting of two regulatory and two catalytic subunits. The regulatory subunit of cAMP-dependent protein kinase (PRKAR2A) is one of the regulatory subunits and the gene is located on chromosome region 3p21.3-p21.2. The expression of PRKAR2A is tightly regulated during spermatogenesis, a significant increase in expression of this gene was also found in the human myometrium during pregnancy.