验证数据展示
经过测试的应用
| Positive WB detected in | U2OS cells, NIH/3T3 cells, HSC-T6 cells, Jurkat cells, HepG2 cells, HeLa cells, LNCaP cells |
| Positive IHC detected in | human colon cancer tissue, mouse colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy PRKAR2A Ready-To-Use IHC Kit. PRKAR2A primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
67751-1-Ig targets PRKAR2A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, rat, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | Human, rat, mouse |
| 文献引用反应性 | human |
| 免疫原 | PRKAR2A fusion protein Ag29893 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | protein kinase, cAMP-dependent, regulatory, type II, alpha |
| 别名 | PKR2, PRKAR2, PRKAR2A |
| 计算分子量 | 43 kDa |
| 观测分子量 | 50 kDa |
| GenBank蛋白编号 | BC002763 |
| 基因名称 | PRKAR2A |
| Gene ID (NCBI) | 5576 |
| RRID | AB_2918520 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P13861 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The effects of cAMP in most tissues and cell types are mainly modulated via protein kinase A, a heterotetrameric protein complex consisting of two regulatory and two catalytic subunits. The regulatory subunit of cAMP-dependent protein kinase (PRKAR2A) is one of the regulatory subunits and the gene is located on chromosome region 3p21.3-p21.2. The expression of PRKAR2A is tightly regulated during spermatogenesis, a significant increase in expression of this gene was also found in the human myometrium during pregnancy.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PRKAR2A antibody 67751-1-Ig | Download protocol |
| IHC protocol for PRKAR2A antibody 67751-1-Ig | Download protocol |
| IF protocol for PRKAR2A antibody 67751-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
