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验证数据展示

  • FC experiment of MCF-7 using CL488-67751

    FC experiment of MCF-7 using CL488-67751

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human PRKAR2A (CL488-67751, Clone:1B2B3) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HeLa using CL488-67751

    IF Staining of HeLa using CL488-67751

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 PRKAR2A antibody (CL488-67751, Clone: 1B2B3 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated PRKAR2A Monoclonal antibody

PRKAR2A Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-67751

产品说明书

CloneNo. 1B2B3

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

PRKAR2, PKR2, cAMP-dependent protein kinase type II-alpha regulatory subunit, 1B2B3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy PRKAR2A Ready-To-Use IHC Kit. PRKAR2A primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67751-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67751 targets PRKAR2A in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 PRKAR2A fusion protein Ag29893 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 protein kinase, cAMP-dependent, regulatory, type II, alpha
别名 PRKAR2, PKR2, cAMP-dependent protein kinase type II-alpha regulatory subunit, 1B2B3
计算分子量 43 kDa
观测分子量50 kDa
GenBank蛋白编号BC002763
基因名称 PRKAR2A
Gene ID (NCBI) 5576
RRIDAB_2923788
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP13861
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The effects of cAMP in most tissues and cell types are mainly modulated via protein kinase A, a heterotetrameric protein complex consisting of two regulatory and two catalytic subunits. The regulatory subunit of cAMP-dependent protein kinase (PRKAR2A) is one of the regulatory subunits and the gene is located on chromosome region 3p21.3-p21.2. The expression of PRKAR2A is tightly regulated during spermatogenesis, a significant increase in expression of this gene was also found in the human myometrium during pregnancy.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 PRKAR2A antibody CL488-67751Download protocol
Standard Protocols
Click here to view our Standard Protocols