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验证数据展示

  • FC experiment of MCF-7 using CL488-67751

    FC experiment of MCF-7 using CL488-67751

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human PRKAR2A (CL488-67751, Clone:1B2B3) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HeLa using CL488-67751

    IF Staining of HeLa using CL488-67751

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 PRKAR2A antibody (CL488-67751, Clone: 1B2B3 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated PRKAR2A Monoclonal antibody

PRKAR2A Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-67751

Print datasheet

CloneNo. 1B2B3

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Synonyms

PRKAR2, PKR2, cAMP-dependent protein kinase type II-alpha regulatory subunit, 1B2B3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488

Size: 
100 μL

¥2800/ 100 μL

浓度: 1000 μg/ml

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy PRKAR2A Ready-To-Use IHC Kit. PRKAR2A primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67751-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67751 targets PRKAR2A in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen PRKAR2A fusion protein Ag29893 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name protein kinase, cAMP-dependent, regulatory, type II, alpha
Synonyms PRKAR2, PKR2, cAMP-dependent protein kinase type II-alpha regulatory subunit, 1B2B3
Calculated Molecular Weight 43 kDa
Observed Molecular Weight50 kDa
GenBank Accession NumberBC002763
Gene Symbol PRKAR2A
Gene ID (NCBI) 5576
RRIDAB_2923788
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP13861
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The effects of cAMP in most tissues and cell types are mainly modulated via protein kinase A, a heterotetrameric protein complex consisting of two regulatory and two catalytic subunits. The regulatory subunit of cAMP-dependent protein kinase (PRKAR2A) is one of the regulatory subunits and the gene is located on chromosome region 3p21.3-p21.2. The expression of PRKAR2A is tightly regulated during spermatogenesis, a significant increase in expression of this gene was also found in the human myometrium during pregnancy.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 PRKAR2A antibody CL488-67751Download protocol
Standard Protocols
Click here to view our Standard Protocols