CoraLite® Plus 488-conjugated PRKCA Recombinant antibody
PRKCA Uni-rAbTM Recombinant Antibody for IF/ICC
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
240926E1
验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy PRKCA Ready-To-Use IHC Kit. PRKCA primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 83840-6-PBS
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
The immunogen of CL488-83840-6 is PRKCA Fusion Protein expressed in E. coli.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | human |
| Immunogen | PRKCA fusion protein Ag1025 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | protein kinase C, alpha |
| Synonyms | |
| Observed Molecular Weight | 77 kDa |
| GenBank Accession Number | BC109274 |
| Gene Symbol | PKC Alpha |
| Gene ID (NCBI) | 5578 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P17252 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. PRKCA is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 PRKCA antibody CL488-83840-6 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |