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pan-PKC Polyclonal antibody
pan-PKC Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA, IF
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat, zebrafish
Applications
WB, IHC, FC (Intra), IP, ELISA, IF
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | K-562 cells, mouse thymus tissue, human peripheral blood platelets, mouse brain tissue, rat brain tissue |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | zebrafish retina |
Positive FC (Intra) detected in | K-562 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:8000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:150-1:600 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
12919-1-AP targets pan-PKC in WB, IHC, IF, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat, zebrafish samples.
Tested Applications | WB, IHC, FC (Intra), IP, ELISA, IF Application Description |
Cited Applications | WB, IHC, IF |
Tested Reactivity | human, mouse, rat, zebrafish |
Cited Reactivity | human, mouse, rat |
Immunogen | pan-PKC fusion protein Ag3591 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | protein kinase C, beta |
Synonyms | PKC beta, PRKCB, PKC-beta, PKC-B, PKCB |
Calculated Molecular Weight | 673 aa, 77 kDa |
Observed Molecular Weight | 77 kDa |
GenBank Accession Number | BC036472 |
Gene Symbol | PRKCB |
Gene ID (NCBI) | 5579 |
RRID | AB_2237469 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P05771 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for pan-PKC antibody 12919-1-AP | Download protocol |
IHC protocol for pan-PKC antibody 12919-1-AP | Download protocol |
IP protocol for pan-PKC antibody 12919-1-AP | Download protocol |
FC protocol for pan-PKC antibody 12919-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
J Hematol Oncol Integrated proteogenomic characterization of urothelial carcinoma of the bladder. | ||
Am J Respir Cell Mol Biol Intermittent Hypoxia Augments Pulmonary Vasoconstrictor Reactivity through PKCβ/Mitochondrial Oxidant Signaling.
| ||
J Cell Mol Med Brucine suppresses colon cancer cells growth via mediating KDR signalling pathway. | ||
Oxid Med Cell Longev p66Shc Mediates Mitochondrial Dysfunction Dependent on PKC Activation in Airway Epithelial Cells Induced by Cigarette Smoke. | ||
J Cell Mol Med Prostaglandin E3 attenuates macrophage-associated inflammation and prostate tumour growth by modulating polarization. | ||
Reprod Biol Endocrinol In vitro fertilization with frozen embryo transfer increased histamine-mediated contractile sensitivity via PKCβ in human umbilical vein |