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CoraLite® Plus 647-conjugated PKC Beta Polyclonal antibody
PKC Beta Polyclonal Antibody for FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
FC (Intra)
Conjugate
CoraLite® Plus 647 Fluorescent Dye
验证数据展示
经过测试的应用
Positive FC (Intra) detected in | K-562 cells |
Planning an IHC experiment? We recommend our IHCeasy PRKCB Ready-To-Use IHC Kit. PRKCB primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 12919-1-AP
推荐稀释比
Application | Dilution |
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Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL647-12919 targets PKC Beta in FC (Intra) applications and shows reactivity with human samples.
Tested Applications | FC (Intra) Application Description |
Tested Reactivity | human |
Immunogen | PKC Beta fusion protein Ag3591 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | protein kinase C, beta |
Synonyms | PRKCB, PKC-beta, PKC-B, PKCB, PKC B |
Calculated Molecular Weight | 673 aa, 77 kDa |
Observed Molecular Weight | 77 kDa |
GenBank Accession Number | BC036472 |
Gene Symbol | PRKCB |
Gene ID (NCBI) | 5579 |
Conjugate | CoraLite® Plus 647 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 654 nm / 674 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P05771 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. Protein kinase Cs (PKCs) are signaling molecules that play key roles in many cellular processes, including secretion, gene expression, proliferation, and muscle contraction. The PKC family is broadly divided into three subgroups: classical, novel, and atypical PKCs. These subgroups differ in both protein sequences and mechanistic requirements for catalytic activity. PKC beta is one of the PKC family members. PKC beta has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. This antibody can recognize PKC beta, PKC alpha, PKC gamma, PKC epsilon and PKC delta.
实验方案
Product Specific Protocols | |
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FC protocol for CL Plus 647 PKC Beta antibody CL647-12919 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |