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CoraLite® Plus 647-conjugated PKC Beta Polyclonal antibody

PKC Beta Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-12919

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Synonyms

PRKCB, PKC-beta, PKC-B, PKCB, PKC B



经过测试的应用

Positive FC (Intra) detected inK-562 cells
Planning an IHC experiment? We recommend our IHCeasy PRKCB Ready-To-Use IHC Kit. PRKCB primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 12919-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-12919 targets PKC Beta in FC (Intra) applications and shows reactivity with human samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human
Immunogen PKC Beta fusion protein Ag3591 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein kinase C, beta
Synonyms PRKCB, PKC-beta, PKC-B, PKCB, PKC B
Calculated Molecular Weight 673 aa, 77 kDa
Observed Molecular Weight 77 kDa
GenBank Accession NumberBC036472
Gene Symbol PRKCB
Gene ID (NCBI) 5579
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP05771
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. Protein kinase Cs (PKCs) are signaling molecules that play key roles in many cellular processes, including secretion, gene expression, proliferation, and muscle contraction. The PKC family is broadly divided into three subgroups: classical, novel, and atypical PKCs. These subgroups differ in both protein sequences and mechanistic requirements for catalytic activity. PKC beta is one of the PKC family members. PKC beta has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. This antibody can recognize PKC beta, PKC alpha, PKC gamma, PKC epsilon and PKC delta.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 PKC Beta antibody CL647-12919Download protocol
Standard Protocols
Click here to view our Standard Protocols