验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy PRKCSH Ready-To-Use IHC Kit. PRKCSH primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 12148-1-AP
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-12148 targets PRKCSH in IF/ICC applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | PRKCSH fusion protein Ag2796 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | protein kinase C substrate 80K-H |
| 别名 | 80K H protein, AGE R2, G19P1, Glucosidase 2 subunit beta, Glucosidase II subunit beta, PCLD, PKCSH, PLD1, PRKCSH |
| 计算分子量 | 80 kDa |
| 观测分子量 | 80 kDa |
| GenBank蛋白编号 | BC013586 |
| 基因名称 | PRKCSH |
| Gene ID (NCBI) | 5589 |
| RRID | AB_3672527 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P14314 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PRKCSH encodes the beta-subunit of glucosidase II, an N-linked glycan-processing enzyme in the endoplasmic reticulum (ER). This protein is an acidic phospho-protein known to be a substrate for protein kinase C. Defects in PRKCSH are a cause of an autosomal dominant polycystic liver disease (PCLD). Glucosidase II is an ER-localized enzyme that contains α and β subunits (Glucosidase IIα and Glucosidase IIβ) which form a defined heterodimeric complex.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 PRKCSH antibody CL488-12148 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |