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PRMT3 Recombinant antibody

PRMT3 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241092H2

Cat no : 83969-6-RR

Print datasheet

Synonyms

Protein arginine N-methyltransferase 3, PRMT 3, HRMT1L3, Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 3, EC:2.1.1.319

验证数据展示

  • WB analysis using 83969-6-RR

    WB analysis using 83969-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83969-6-RR (PRMT3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 83969-6-RR

    IHC staining of mouse liver using 83969-6-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83969-6-RR (PRMT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 83969-6-RR

    IF Staining of HepG2 using 83969-6-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PRMT3 antibody (83969-6-RR, Clone: 241092H2 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of MCF-7 using 83969-6-RR

    FC experiment of MCF-7 using 83969-6-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug PRMT3 Recombinant antibody (83969-6-RR, Clone:241092H2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inHepG2 cells, HuH-7 cells, SW480 cells, HEK-293 cells
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83969-6-RR is PRMT3 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse
Immunogen PRMT3 fusion protein Ag13466 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name protein arginine methyltransferase 3
Synonyms Protein arginine N-methyltransferase 3, PRMT 3, HRMT1L3, Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 3, EC:2.1.1.319
Calculated Molecular Weight 531 aa, 60 kDa
Observed Molecular Weight60-68 kDa
GenBank Accession NumberBC037544
Gene Symbol PRMT3
Gene ID (NCBI) 10196
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO60678
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PRMT3(protein arginine methyltransferase 3) regulates protein functions by introducing asymmetric dimethylation marks at the arginine residues in proteins.

实验方案

Product Specific Protocols
WB protocol for PRMT3 antibody 83969-6-RRDownload protocol
IHC protocol for PRMT3 antibody 83969-6-RRDownload protocol
IF protocol for PRMT3 antibody 83969-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols